Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,700,633	T→C	V122V (GTA→GTG)	acpS ←	holo‑[acyl‑carrier‑protein] synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,700,633	0	T	C	100.0%	61.4 / NA	18	V122V (GTA→GTG)	acpS	holo‑[acyl‑carrier‑protein] synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/6);  total (12/6)

AACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGT  >  NC_000913/2700561‑2700708                                                                         |                                                                            aaCCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGCATTATTTTTAACTTTCAATAATCACCGTgg                                                                      >  2:482130/1‑80 (MQ=255)           ccTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAg                                                           >  2:482134/1‑81 (MQ=255)                         gggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGc                                             >  1:482139/1‑81 (MQ=255)                              ttACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGc                                         <  1:482115/80‑1 (MQ=255)                              ttACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGc                                         <  1:482114/80‑1 (MQ=255)                               tACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCg                                        <  1:482116/80‑1 (MQ=255)                                     ttAAAGAGAACGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATg                                 >  2:482147/1‑81 (MQ=255)                                               cGGTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCa                       <  1:482117/81‑1 (MQ=255)                                                ggTATTATTTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCaa                      <  1:482118/81‑1 (MQ=255)                                                    ttattTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGcc                  >  1:482153/1‑81 (MQ=255)                                                    ttattTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGc                   <  1:482119/80‑1 (MQ=255)                                                      attTTTAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGcc                  >  1:482143/1‑79 (MQ=255)                                                          ttAACTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCtt            >  2:482146/1‑81 (MQ=255)                                                            aaCTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCtttt          >  1:482149/1‑81 (MQ=255)                                                             aCTTTCAATAATCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTc         >  2:482156/1‑81 (MQ=255)                                                                   aataatCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAg   >  1:482150/1‑81 (MQ=255)                                                                   aataatCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAg   >  1:482145/1‑81 (MQ=255)                                                                    ataatCACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGt  >  1:482136/1‑81 (MQ=255)                                                                         |                                                                            AACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGT  >  NC_000913/2700561‑2700708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑