Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,453	G→A	intergenic (+10/+38)	ung → / ← yfiF	uracil‑DNA glycosylase/putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,453	0	G	A	100.0%	68.8 / NA	20	intergenic (+10/+38)	ung/yfiF	uracil‑DNA glycosylase/putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

AACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACG  >  NC_000913/2717388‑2717530                                                                  |                                                                              aacGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATgg                                                                  >  1:485663/1‑79 (MQ=255) aacGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATgg                                                                  >  1:485672/1‑79 (MQ=255)      ggCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGtt                                                           >  1:485655/1‑81 (MQ=255)       gCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGtt                                                           <  2:485635/80‑1 (MQ=255)             cGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGcccccc                                                    >  1:485733/1‑81 (MQ=255)             cGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCAccc                                                    <  1:485636/81‑1 (MQ=255)              gCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCg                                                   <  1:485637/81‑1 (MQ=255)                 gATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCt                                                <  2:485638/81‑1 (MQ=255)                  aTTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCt                                                >  2:485669/1‑80 (MQ=255)                      aCTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGAtt                                            >  2:485668/1‑80 (MQ=255)                                    ttACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTc                             <  1:485639/81‑1 (MQ=255)                                      aCCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCt                            >  1:485684/1‑80 (MQ=255)                                      aCCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCt                            >  2:485685/1‑80 (MQ=255)                                       ccGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTg                           <  2:485640/80‑1 (MQ=255)                                            agagagTGAGTAAATTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGcca                     >  2:485667/1‑81 (MQ=255)                                                       aaaTTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCAcc            >  2:485681/1‑79 (MQ=255)                                                        aaTTTGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCaa          <  2:485641/80‑1 (MQ=255)                                                          tttGCGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCa       <  1:485642/81‑1 (MQ=255)                                                              cGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAAc   >  1:485691/1‑81 (MQ=255)                                                              cGGGAAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAAc   <  2:485643/81‑1 (MQ=255)                                                               gggaAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACg  >  1:485665/1‑81 (MQ=255)                                                                  |                                                                              AACGTGGCGAGACGCCGATTGACTGGATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCGGATGGCAGAGTTGCCACCCGGCTGATTTATCAGGCTTTATTCTGACGCCACCATTCACCAAGCAAAACG  >  NC_000913/2717388‑2717530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑