Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,817	C→T	A238T (GCG→ACG)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,817	0	C	T	100.0%	113.2 / NA	31	A238T (GCG→ACG)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/17);  total (14/17)

ATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCAT  >  NC_000913/2717740‑2717889                                                                              |                                                                         atcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCTCCGCCTTCTGCGGTACGGATAGCCGtccc                                                                       >  1:485747/1‑81 (MQ=255) atcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtccc                                                                       <  2:485703/81‑1 (MQ=255)  tcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcccc                                                                      <  2:485704/81‑1 (MQ=255)    cAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCg                                                                     <  2:485705/80‑1 (MQ=255)           ttAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcag                                                              >  2:485755/1‑80 (MQ=255)              aCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagcag                                                           <  2:485706/80‑1 (MQ=255)                        cGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                >  1:485751/1‑81 (MQ=255)                        cGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                >  1:485758/1‑81 (MQ=255)                             gTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcacc                                           <  1:485707/81‑1 (MQ=255)                                   ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGac                                      <  2:485709/80‑1 (MQ=255)                                   ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGac                                      <  2:485708/80‑1 (MQ=255)                                    gCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGacac                                    <  1:485710/81‑1 (MQ=255)                                     cTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACAcc                                   >  2:485762/1‑81 (MQ=255)                                       gAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                 >  2:485765/1‑81 (MQ=255)                                        aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCttt                                >  2:485767/1‑81 (MQ=255)                                           gTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCAc                             >  1:485768/1‑81 (MQ=255)                                              ctctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGcc                          <  2:485711/81‑1 (MQ=255)                                              ctctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGcc                          <  1:485712/81‑1 (MQ=255)                                                ctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                         >  1:485756/1‑80 (MQ=255)                                                    gccgccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTg                    >  1:485753/1‑81 (MQ=255)                                                       gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgc                 <  1:485713/81‑1 (MQ=255)                                                       gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgc                 >  1:485766/1‑81 (MQ=255)                                                        ccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                >  2:485759/1‑81 (MQ=255)                                                         cTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcgc               <  1:485715/81‑1 (MQ=255)                                                         cTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcgc               <  2:485714/81‑1 (MQ=255)                                                               cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt           <  2:485717/79‑1 (MQ=255)                                                               cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt           <  2:485718/79‑1 (MQ=255)                                                               cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgc         <  2:485716/81‑1 (MQ=255)                                                               cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgc         >  2:485757/1‑81 (MQ=255)                                                                ggTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg        >  1:485760/1‑81 (MQ=255)                                                                 gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcgc       <  1:485719/81‑1 (MQ=255)                                                                     ggATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatca   <  1:485720/81‑1 (MQ=255)                                                                      gATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat  <  1:485721/81‑1 (MQ=255)                                                                      gATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat  >  1:485774/1‑81 (MQ=255)                                                                              |                                                                         ATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCAT  >  NC_000913/2717740‑2717889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑