Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,229	C→A	L100F (TTG→TTT)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,229	0	C	A	100.0%	127.6 / NA	36	L100F (TTG→TTT)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/22);  total (14/22)

CGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCG  >  NC_000913/2718151‑2718305                                                                               |                                                                             cGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGaaa                                                                            >  1:485841/1‑81 (MQ=255)  gCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGaaa                                                                            <  1:485809/80‑1 (MQ=255)  gCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGaaa                                                                            >  2:485854/1‑80 (MQ=255)   cTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAaca                                                                          <  2:485810/81‑1 (MQ=255)    ttCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAacac                                                                         >  1:485850/1‑81 (MQ=255)      ccGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACtt                                                                       >  2:485848/1‑81 (MQ=255)       cGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTc                                                                      <  2:485811/81‑1 (MQ=255)          aCGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCgg                                                                   <  2:485813/81‑1 (MQ=255)          aCGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCgg                                                                   <  2:485812/81‑1 (MQ=255)           cGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGa                                                                  <  2:485815/81‑1 (MQ=255)           cGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGa                                                                  <  2:485814/81‑1 (MQ=255)            ggCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGAt                                                                 >  2:485858/1‑81 (MQ=255)              ctctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCa                                                               <  1:485816/81‑1 (MQ=255)                   gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATaa                                                           >  1:485868/1‑80 (MQ=255)                    aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATaaaa                                                         >  1:485864/1‑81 (MQ=255)                    aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATaaaa                                                         <  2:485817/81‑1 (MQ=255)                      aaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACt                                                       >  2:485877/1‑81 (MQ=255)                      aaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACt                                                       >  1:485853/1‑81 (MQ=255)                         tGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACtttt                                                    <  1:485818/81‑1 (MQ=255)                                cATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTg                                             <  2:485819/81‑1 (MQ=255)                                    cATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATg                                          <  1:485820/80‑1 (MQ=255)                                     aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGcc                                        <  2:485821/81‑1 (MQ=255)                                     aTTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGcc                                        <  1:485822/81‑1 (MQ=255)                                       tttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCAc                                      >  1:485852/1‑81 (MQ=255)                                        ttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCAcc                                     >  1:485857/1‑81 (MQ=255)                                          cGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGt                                   >  1:485875/1‑81 (MQ=255)                                           gCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGt                                   <  2:485823/80‑1 (MQ=255)                                                aGACGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCgg                             >  2:485861/1‑81 (MQ=255)                                                  aCGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGcc                           >  2:485865/1‑81 (MQ=255)                                                  aCGCGGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGcc                           <  2:485824/81‑1 (MQ=255)                                                     cgGGTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCttt                        <  1:485825/81‑1 (MQ=255)                                                       ggTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTc                      <  2:485826/81‑1 (MQ=255)                                                        gTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTcc                     <  2:485828/81‑1 (MQ=255)                                                        gTTTCTTCCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTcc                     <  1:485827/81‑1 (MQ=255)                                                           tcttcCGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCgg                   <  1:485829/80‑1 (MQ=255)                                                                         acgacgAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCgg    <  2:485831/81‑1 (MQ=255)                                                                         acgacgAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCgg    >  1:485862/1‑81 (MQ=255)                                                                         acgacgAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCg     <  2:485830/80‑1 (MQ=255)                                                                           gacgAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGgcg  >  2:485860/1‑81 (MQ=255)                                                                               |                                                                             CGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCG  >  NC_000913/2718151‑2718305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑