Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,430	C→T	G33G (GGG→GGA)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,430	0	C	T	100.0%	105.8 / NA	30	G33G (GGG→GGA)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/17);  total (13/17)

GGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT  >  NC_000913/2718352‑2718506                                                                               |                                                                             gggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTtcc                                                                            <  2:485853/81‑1 (MQ=255)       aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTAcg                                                                      <  1:485854/81‑1 (MQ=255)           tGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTAcgcg                                                                    <  2:485855/79‑1 (MQ=255)             tttGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGtt                                                                <  2:485856/81‑1 (MQ=255)               tGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTtg                                                               <  2:485857/80‑1 (MQ=255)                 tCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTgg                                                            >  1:485914/1‑81 (MQ=255)                   aTCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTggg                                                           <  1:485858/80‑1 (MQ=255)                   aTCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTggg                                                           >  1:485913/1‑80 (MQ=255)                     cgcgGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTAc                                                        >  1:485918/1‑81 (MQ=255)                        gggcggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGtt                                                     >  2:485904/1‑81 (MQ=255)                          gcggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTa                                                   >  1:485907/1‑81 (MQ=255)                            ggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTAt                                                  >  2:485921/1‑80 (MQ=255)                            ggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATc                                                 <  2:485859/81‑1 (MQ=255)                               gcgACGGCCACCGTCAGCACGAGATTTTCCTGGGCGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                              <  1:485860/81‑1 (MQ=255)                                 gACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAat                                            >  2:485903/1‑81 (MQ=255)                                   cGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatca                                          <  1:485861/81‑1 (MQ=255)                                    ggCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcat                                         >  2:485906/1‑81 (MQ=255)                                     gCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatc                                        <  2:485862/81‑1 (MQ=255)                                       cACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatcat                                      >  1:485910/1‑81 (MQ=255)                                         ccGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                    <  1:485863/81‑1 (MQ=255)                                           gTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACt                                  >  1:485912/1‑81 (MQ=255)                                               gcgcGAGATTTTCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                              <  2:485864/78‑1 (MQ=255)                                                         ttCCTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATc                     <  1:485865/80‑1 (MQ=255)                                                           ccTGGACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                  >  1:485902/1‑81 (MQ=255)                                                              ggACGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACttt                >  1:485911/1‑80 (MQ=255)                                                                aCGCCCGCCCCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAc             <  1:485866/81‑1 (MQ=255)                                                                   cccgcccCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCtt          <  2:485867/81‑1 (MQ=255)                                                                   cccgcccCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCtt          <  2:485868/81‑1 (MQ=255)                                                                      gcccCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGcc       >  2:485916/1‑81 (MQ=255)                                                                      gcccCCTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGcc       <  1:485869/81‑1 (MQ=255)                                                                           cTTTTCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt  <  2:485870/81‑1 (MQ=255)                                                                               |                                                                             GGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT  >  NC_000913/2718352‑2718506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑