Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,767	T→C	L14P (CTT→CCT)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,767	0	T	C	100.0%	120.6 / NA	35	L14P (CTT→CCT)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

GGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCGGGAGACGC  >  NC_000913/2722692‑2722844                                                                            |                                                                              ggACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGcc                                                                           >  2:486885/1‑80 (MQ=255)  gACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGccc                                                                          >  1:486894/1‑80 (MQ=255)    cccTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAAc                                                                       <  1:486848/81‑1 (MQ=255)           aTGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTAccc                                                                  >  2:486888/1‑79 (MQ=255)             gAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTAccc                                                                  >  2:486892/1‑77 (MQ=255)             gAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTAccc                                                                  >  2:486884/1‑77 (MQ=255)              aaCAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGa                                                              <  1:486849/80‑1 (MQ=255)                  gagaAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTtct                                                         >  1:486879/1‑81 (MQ=255)                   agaagaAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTtctc                                                        <  2:486850/81‑1 (MQ=255)                     aagaaATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCaa                                                      >  1:486890/1‑81 (MQ=255)                        aaaTGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCa                                                   <  1:486851/81‑1 (MQ=255)                          aTGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGt                                                 <  1:486852/81‑1 (MQ=255)                          aTGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGt                                                 >  2:486891/1‑81 (MQ=255)                             cACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTtga                                              >  2:486883/1‑81 (MQ=255)                               cTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTtgatg                                            <  2:486853/81‑1 (MQ=255)                                 gtgATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTgatgat                                          >  2:486903/1‑81 (MQ=255)                                    aTGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGt                                        <  1:486854/80‑1 (MQ=255)                                         gtCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGAttt                                  >  1:486893/1‑81 (MQ=255)                                         gtCAAAATTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGAttt                                  >  2:486889/1‑81 (MQ=255)                                            aaaaTTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCtt                               <  2:486856/81‑1 (MQ=255)                                            aaaaTTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCtt                               >  2:486909/1‑81 (MQ=255)                                            aaaaTTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCt                                <  2:486855/80‑1 (MQ=255)                                             aaaTTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCttt                              <  2:486857/81‑1 (MQ=255)                                               aTTTAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTa                            >  1:486898/1‑81 (MQ=255)                                                 ttAAGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACg                          >  2:486896/1‑81 (MQ=255)                                                   aaGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCt                        >  2:486910/1‑81 (MQ=255)                                                    aGCGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTc                       >  2:486882/1‑81 (MQ=255)                                                      cGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTccc                     <  1:486859/81‑1 (MQ=255)                                                      cGTAATAAACATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTccc                     <  1:486858/81‑1 (MQ=255)                                                               cATCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTc            <  2:486860/81‑1 (MQ=255)                                                                aTCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTcc           >  1:486887/1‑81 (MQ=255)                                                                 tCAACAACACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCg          <  1:486861/81‑1 (MQ=255)                                                                   aacaacACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCggg        <  1:486862/81‑1 (MQ=255)                                                                   aacaacACCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCgg         >  2:486886/1‑80 (MQ=255)                                                                         acCCTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCGGGAGACgc  >  1:486962/1‑81 (MQ=255)                                                                            |                                                                              GGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCGGGAGACGC  >  NC_000913/2722692‑2722844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑