Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,198	T→C	K111K (AAA→AAG)	rplS ←	50S ribosomal subunit protein L19

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,198	0	T	C	100.0%	121.4 / NA	34	K111K (AAA→AAG)	rplS	50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

AGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTT  >  NC_000913/2744121‑2744272                                                                              |                                                                           aGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCctt                                                                          >  1:491323/1‑80 (MQ=255)  gATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGa                                                                        >  1:491312/1‑81 (MQ=255)      aaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATAc                                                                     <  2:491279/80‑1 (MQ=255)       aaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATAc                                                                     >  1:491319/1‑79 (MQ=255)        aaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGag                                                                  >  2:491336/1‑81 (MQ=255)        aaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGag                                                                  >  2:491329/1‑81 (MQ=255)          aGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGagca                                                                <  1:491280/81‑1 (MQ=255)           gggCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGagcag                                                               <  1:491281/81‑1 (MQ=255)                agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTa                                                          >  2:491324/1‑81 (MQ=255)                 gccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTAc                                                         >  1:491331/1‑81 (MQ=255)                    aaTTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAg                                                      >  1:491335/1‑81 (MQ=255)                     aTTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGt                                                     <  2:491282/81‑1 (MQ=255)                       tGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTAc                                                   >  2:491333/1‑81 (MQ=255)                        ggCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTAc                                                   <  2:491283/80‑1 (MQ=255)                            aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCa                                              >  2:491325/1‑81 (MQ=255)                                 ttcttAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAg                                         >  1:491334/1‑81 (MQ=255)                                  tcttAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAgg                                        >  1:491326/1‑81 (MQ=255)                                     tAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgtag                                     >  1:491330/1‑81 (MQ=255)                                     tAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgtag                                     >  1:491337/1‑81 (MQ=255)                                             tGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGttt                             <  2:491284/81‑1 (MQ=255)                                              gTCGCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGtt                              >  2:491317/1‑79 (MQ=255)                                                 gCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCt                         <  1:491286/81‑1 (MQ=255)                                                 gCTTAAGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCt                         <  1:491285/81‑1 (MQ=255)                                                     aaGCGAAACCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTAc                     >  1:491332/1‑81 (MQ=255)                                                      aGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTAc                     >  1:491315/1‑80 (MQ=255)                                                      aGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTAc                     <  1:491287/80‑1 (MQ=255)                                                      aGCGAAATCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTAc                     >  2:491340/1‑80 (MQ=255)                                                          aaaTCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGaa                  >  2:491320/1‑79 (MQ=255)                                                           aaTCTTAGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAg               <  1:491288/81‑1 (MQ=255)                                                                 aGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGcacca          <  2:491290/80‑1 (MQ=255)                                                                 aGTTAAGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGca             <  2:491289/77‑1 (MQ=255)                                                                     aaGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCacgac      <  2:491291/80‑1 (MQ=255)                                                                      aGACGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGt    <  1:491292/81‑1 (MQ=255)                                                                        aCGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGttt  >  1:491352/1‑81 (MQ=255)                                                                        aCGCTCCTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGttt  <  1:491293/81‑1 (MQ=255)                                                                              |                                                                           AGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTT  >  NC_000913/2744121‑2744272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑