Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,880	T→C	Y521Y (TAT→TAC)	yfjW →	CP4‑57 prophage; uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,880	0	T	C	100.0%	20.1 / NA	7	Y521Y (TAT→TAC)	yfjW	CP4‑57 prophage; uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/3);  total (4/3)

CAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTT  >  NC_000913/2774802‑2774944                                                                               |                                                                 gcaaaaaaTCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAacttactt                                                                >  1:496983/3‑81 (MQ=255)           aaaaTATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTACTTGCAATCTAAg                                                      >  2:496990/1‑81 (MQ=255)             aaTATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTACTTGCAATCTAAGCt                                                    <  1:496971/81‑1 (MQ=255)                     gCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTACTTGCAATCTAAGCTAGAAAttt                                            >  1:496981/1‑81 (MQ=255)                                   gTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTACTTGCAATCTAAGCTAGAAATTTTTGGttctt                                   <  1:496972/76‑1 (MQ=255)                                                    gATAATGAACCTTCGTAAAATAACTTACTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGta             >  1:496988/1‑81 (MQ=255)                                                            aCCTTCGTAAAATAACTTACTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTg     <  2:496973/81‑1 (MQ=255)                                                               ttCGTAAAATAACTTACTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGtt  >  1:496989/1‑81 (MQ=255)                                                                               |                                                                 CAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTT  >  NC_000913/2774802‑2774944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑