Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,044	A→G	intergenic (+32/‑57)	ygbM → / → ygbN	putative 2‑oxo‑tetronate isomerase YgbM/putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,044	0	A	G	100.0%	138.7 / NA	40	intergenic (+32/‑57)	ygbM/ygbN	putative 2‑oxo‑tetronate isomerase YgbM/putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/25);  total (15/25)

GTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTAT  >  NC_000913/2864966‑2865122                                                                               |                                                                               gTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCa                                                                              >  2:516620/1‑81 (MQ=255)     ccTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATccc                                                                          <  2:516579/81‑1 (MQ=255)     ccTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATccc                                                                          <  2:516578/81‑1 (MQ=255)        cACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATccccc                                                                        >  1:516631/1‑80 (MQ=255)            gaagaaGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGATAAAGCCCTAATTACAACGTACCCATGCATCCCCCCATCt                                                                   <  1:516580/81‑1 (MQ=255)              agaagGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTAc                                                                 <  2:516581/81‑1 (MQ=255)                aagGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACgg                                                               <  2:516582/81‑1 (MQ=255)                  gggCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGAt                                                             <  2:516583/81‑1 (MQ=255)                    gCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATgg                                                           <  2:516584/81‑1 (MQ=255)                         gCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAg                                                      >  1:516644/1‑81 (MQ=255)                          cTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAgg                                                     <  2:516585/81‑1 (MQ=255)                             gTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGtt                                                   >  2:516654/1‑80 (MQ=255)                                tGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAAc                                                >  1:516619/1‑80 (MQ=255)                                 gACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACta                                              >  1:516629/1‑81 (MQ=255)                                  aCGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGG‑TAACtata                                            <  2:516586/81‑1 (MQ=255)                                   cGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACtata                                            <  1:516587/81‑1 (MQ=255)                                     ccTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAAc                                          <  2:516588/81‑1 (MQ=255)                                         gcgcTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACttt                                       <  1:516589/80‑1 (MQ=255)                                          cgcTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCa                                     <  1:516590/81‑1 (MQ=255)                                           gcTAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAg                                    <  2:516591/81‑1 (MQ=255)                                             tAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGa                                   >  2:516633/1‑80 (MQ=255)                                             tAATTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGa                                   >  1:516639/1‑80 (MQ=255)                                              aaTTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGa                                   >  1:516632/1‑79 (MQ=255)                                               aTTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAg                                >  2:516634/1‑81 (MQ=255)                                               aTTCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAg                                <  2:516592/81‑1 (MQ=255)                                                ttCGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAgg                               <  2:516593/81‑1 (MQ=255)                                                  cGTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAggg                              <  2:516594/80‑1 (MQ=255)                                                   gTTAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGt                             <  2:516595/80‑1 (MQ=255)                                                    ttAAATCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGttt                           <  2:516596/81‑1 (MQ=255)                                                      aaaTCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTc                          <  1:516597/80‑1 (MQ=255)                                                         tCCCTAATTACAACGTACCCATGCATCCCCCCATCTACGGATAGGGTAGGTTAACTATAACTGTCAGACAGGGTTTCCCAt                      <  2:516598/81‑1 (MQ=255)                                                           ccTAATTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGt                    <  2:516599/81‑1 (MQ=255)                                                               aTTACAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCca                 >  1:516630/1‑80 (MQ=255)                                                                   cAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAAt             <  2:516600/80‑1 (MQ=255)                                                                   cAACGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAAt             <  2:516601/80‑1 (MQ=255)                                                                     aCGTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTa           >  2:516641/1‑80 (MQ=255)                                                                       gTACCCATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACAt        >  1:516672/1‑81 (MQ=255)                                                                           ccATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGtt    <  1:516602/81‑1 (MQ=255)                                                                            cATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTa   >  1:516645/1‑81 (MQ=255)                                                                            cATGCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAA‑TACATTGTTAt  >  2:516648/1‑81 (MQ=255)                                                                               |                                                                               GTGGCCTCACCGAAGAAGGGCTTGGCTGGTTTGACGCCTGGCGCTAATTCGTTAAATCCCTAATTACAACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTAT  >  NC_000913/2864966‑2865122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑