| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,882,617 | T→C | A7A (GCA→GCG) | casB ← | type I‑E CRISPR system Cascade subunit CasB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,882,617 | 0 | T | C | 95.7% | 75.3 / NA | 23 | A7A (GCA→GCG) | casB | type I‑E CRISPR system Cascade subunit CasB |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (10/12); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGCGCG > NC_000913/2882540‑2882692 | tcatcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCa > 2:520348/1‑81 (MQ=255) atcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCAt > 1:520353/1‑80 (MQ=255) atcaGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATc < 1:520323/81‑1 (MQ=255) aaTTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATg > 2:520350/1‑81 (MQ=255) aTTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATgg < 1:520324/81‑1 (MQ=255) ttGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGcc > 2:520354/1‑81 (MQ=255) cgcACATGATCCATTATCCTGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCcctc > 2:520359/1‑81 (MQ=255) acaTGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCtt < 2:520325/81‑1 (MQ=255) ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTaaa < 2:520327/80‑1 (MQ=255) ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAAt < 1:520328/81‑1 (MQ=255) ttgCCAGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAAt < 1:520326/81‑1 (MQ=255) aGGCTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTg > 1:520362/1‑81 (MQ=255) cTCGATATAAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGtat < 2:520329/81‑1 (MQ=255) tataAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGt < 2:520330/81‑1 (MQ=255) taAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTgg < 1:520332/81‑1 (MQ=255) taAAGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTgg > 2:520372/1‑81 (MQ=255) taAAGCCATCGCATCAATTTCATCAGCCATATGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTg < 2:520331/80‑1 (MQ=255) aaGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgcg < 2:520333/81‑1 (MQ=255) aaGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgc < 1:520334/80‑1 (MQ=255) aGCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGgcgc > 1:520368/1‑81 (MQ=255) gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg > 1:520358/1‑81 (MQ=255) gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg > 1:520357/1‑81 (MQ=255) gCCATCGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGcgcg > 2:520361/1‑81 (MQ=255) | TCATCAGGTTCTGAAACACGTCTAATTTGCGCACATGATCCATTATCCAGTTGTTGCCAGGCTCGATATAAAGCCATTGCATCAATTTCATCAGCCATTTGATGGCCCTCCTTGCGGTTTTAACTCCCGTAAATGTTTGTATAGCGTGGCGCG > NC_000913/2882540‑2882692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |