Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,883,008	C→A	V377V (GTG→GTT)	casA ←	type I‑E CRISPR system Cascade subunit CasA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,883,008	0	C	A	100.0%	58.1 / NA	17	V377V (GTG→GTT)	casA	type I‑E CRISPR system Cascade subunit CasA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/8);  total (9/8)

AACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGC  >  NC_000913/2882931‑2883076                                                                              |                                                                     aaCCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATaaca                                                                   >  1:520412/1‑81 (MQ=255)        cTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCg                                                             >  2:520411/1‑80 (MQ=255)          gCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTAt                                                          >  2:520417/1‑81 (MQ=255)           cAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTa                                                           <  1:520380/79‑1 (MQ=255)             aaGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTAttgt                                                       >  2:520408/1‑81 (MQ=255)                gTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGc                                                    >  1:520418/1‑81 (MQ=255)                            ttGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGAtt                                         >  2:520424/1‑80 (MQ=255)                                         ttttATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAAcaca                            <  1:520383/80‑1 (MQ=255)                                         ttttATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAAcaca                            <  1:520381/80‑1 (MQ=255)                                         ttttATATCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACAcat                           <  1:520382/81‑1 (MQ=255)                                            tataTCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACAcatcat                        >  1:520420/1‑81 (MQ=255)                                                tCCCAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACg                    <  1:520384/81‑1 (MQ=255)                                                 cccAAACCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGc                   >  2:520414/1‑81 (MQ=255)                                                    aaaCCAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGt                <  1:520385/81‑1 (MQ=255)                                                       ccAACAGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCa             <  2:520386/81‑1 (MQ=255)                                                            aGTCACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGaa         >  1:520415/1‑80 (MQ=255)                                                               cACTATTTCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGaa         >  2:520423/1‑77 (MQ=255)                                                                    tttCGTTTATAACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGc  <  1:520387/79‑1 (MQ=255)                                                                              |                                                                     AACCCTTCTGCAAAGGTATATAACGCCTTGCGTAAGGCTGTTTTATATCCCAAACCAACAGTCACTATTTCGTTTATCACATTGCCGTATTGTTGCCACCCCTGATTAAACATCAACACATCATGACGCCGTTCAAGAATAGATGC  >  NC_000913/2882931‑2883076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑