Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,903	G→A	intergenic (+231/‑326)	ppnN → / → sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,903	0	G	A	100.0%	99.5 / NA	28	intergenic (+231/‑326)	ppnN/sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCT  >  NC_000913/2927825‑2927979                                                                               |                                                                             cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAAt                                                                            <  1:529586/81‑1 (MQ=255)       tttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTc                                                                      <  1:529587/81‑1 (MQ=255)       tttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTc                                                                      <  2:529588/81‑1 (MQ=255)        ttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAAtttttt                                                                       >  1:529614/1‑79 (MQ=255)                 ttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAAcc                                                             >  1:529616/1‑80 (MQ=255)                    aTAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACa                                                         >  1:529624/1‑81 (MQ=255)                     tAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACat                                                        <  1:705128/81‑1 (MQ=255)                           gTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTg                                                  >  1:529613/1‑81 (MQ=255)                                 tATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGcc                                            >  2:529621/1‑81 (MQ=255)                                   tCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGccc                                           <  2:529589/80‑1 (MQ=255)                                     gCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCg                                         <  1:529590/80‑1 (MQ=255)                                        aTGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCgg                                        >  2:529625/1‑78 (MQ=255)                                         tGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaa                                      <  1:529591/79‑1 (MQ=255)                                              aaGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGt                                 >  2:529631/1‑79 (MQ=255)                                               aGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAg                              <  1:529592/81‑1 (MQ=255)                                                 aaaTTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAt                            >  2:529619/1‑81 (MQ=255)                                                     tATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCg                        >  2:529629/1‑81 (MQ=255)                                                         gCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTa                    <  2:529593/81‑1 (MQ=255)                                                           cATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt                  >  1:529612/1‑81 (MQ=255)                                                            aTGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATg                 >  1:529620/1‑81 (MQ=255)                                                                 tAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCa             >  2:529618/1‑80 (MQ=255)                                                                   aCTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCaaa           >  2:529628/1‑80 (MQ=255)                                                                      aaaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt       >  2:529615/1‑81 (MQ=255)                                                                       aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAatat        <  1:529595/79‑1 (MQ=255)                                                                       aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt       <  2:529594/80‑1 (MQ=255)                                                                       aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg      <  2:529596/81‑1 (MQ=255)                                                                          gTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGccc   >  2:529636/1‑81 (MQ=255)                                                                            aTTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt  >  1:529623/1‑80 (MQ=255)                                                                               |                                                                             CAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCT  >  NC_000913/2927825‑2927979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑