Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,077	T→C	V262V (GTA→GTG)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,077	0	T	C	100.0%	165.7 / NA	46	V262V (GTA→GTG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

GCTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAAC  >  NC_000913/2973000‑2973149                                                                              |                                                                         gCTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCAc                                                                        >  2:539826/1‑80 (MQ=255)  cTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGccacca                                                                      >  2:539809/1‑81 (MQ=255)   tGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCAt                                                                     >  1:539820/1‑81 (MQ=255)    ggCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATc                                                                    >  2:539821/1‑81 (MQ=255)    ggCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATc                                                                    >  2:539825/1‑81 (MQ=255)        tACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGt                                                                <  2:539757/81‑1 (MQ=255)          cAGCGTGACACTGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTTCTCCAACCACGATACCAATCGCCACCATCGCGttt                                                              >  2:539818/1‑80 (MQ=255)            gCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTgag                                                            <  1:539758/81‑1 (MQ=255)               tGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATa                                                         <  1:539759/81‑1 (MQ=255)                gACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAg                                                        >  2:539813/1‑81 (MQ=255)                gACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAg                                                        >  1:539827/1‑81 (MQ=255)                   acGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTg                                                     >  2:539830/1‑81 (MQ=255)                   acGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTg                                                     >  1:539815/1‑81 (MQ=255)                       tttCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCg                                                 <  2:539760/81‑1 (MQ=255)                         tCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGt                                                <  1:539761/80‑1 (MQ=255)                           cAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGc                                             <  1:539762/81‑1 (MQ=255)                            aGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCg                                            <  1:539763/81‑1 (MQ=255)                              cataactaacTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGAGCGTAGCGtt                                          <  2:539764/79‑1 (MQ=255)                                taactaacTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTAt                                        >  1:539812/1‑81 (MQ=255)                                 aactaactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATc                                       >  1:539838/1‑81 (MQ=255)                                  actaactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATc                                       <  1:539765/80‑1 (MQ=255)                                   ctaactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCgg                                     >  2:539831/1‑81 (MQ=255)                                   ctaactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCgg                                     >  2:539836/1‑81 (MQ=255)                                   ctaactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCgg                                     <  1:539766/81‑1 (MQ=255)                                     aactTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGt                                    >  1:539814/1‑80 (MQ=255)                                      actTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTa                                   <  1:539767/80‑1 (MQ=255)                                      actTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTa                                   <  2:539768/80‑1 (MQ=255)                                          cGCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATg                                <  2:539769/79‑1 (MQ=255)                                           gCTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGcc                              <  2:539770/80‑1 (MQ=255)                                            cTGCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCa                            <  2:539771/81‑1 (MQ=255)                                              gCCGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgc                          >  1:539823/1‑81 (MQ=255)                                               ccGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgcg                         <  2:539772/81‑1 (MQ=255)                                               ccGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAgc                          <  2:539774/80‑1 (MQ=255)                                               ccGCACCTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAg                           <  1:539773/79‑1 (MQ=255)                                                   aCCTGCGCCAACCACGATACCAATCGCCACCATCACGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCAc                     <  1:539775/81‑1 (MQ=255)                                                    ccTGCGCCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCAcc                    >  1:539840/1‑81 (MQ=255)                                                       gcgcCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCgg                  >  1:539824/1‑80 (MQ=255)                                                         gcCAACCACGATACCAATCGCCACCATCGCGTTTAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGTTAc               >  1:539841/1‑81 (MQ=255)                                                         gcCAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAc               <  1:539776/81‑1 (MQ=255)                                                           cAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAccc             <  1:539778/81‑1 (MQ=255)                                                           cAACCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTAccc             <  2:539777/81‑1 (MQ=255)                                                            aaCCACGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCca            <  2:539779/81‑1 (MQ=255)                                                              ccACGATACCAATCGCCACTATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCca            >  1:539833/1‑79 (MQ=255)                                                                 cGATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCa       >  1:539804/1‑81 (MQ=255)                                                                  gATACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCAc      >  1:539851/1‑81 (MQ=255)                                                                    tACCAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCa    <  1:539780/81‑1 (MQ=255)                                                                      ccAATCGCCACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAAc  <  2:539781/81‑1 (MQ=255)                                                                              |                                                                         GCTGGCATACAGCGTGACACGGTTTCCAGCGTAACTAACTTCGCTGCCGCACCTGCGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAAC  >  NC_000913/2973000‑2973149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑