Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,002	C→T	S259F (TCT→TTT)	rclR →	DNA‑binding transcriptional activator RclR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,002	0	C	T	100.0%	85.0 / NA	24	S259F (TCT→TTT)	rclR	DNA‑binding transcriptional activator RclR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/17);  total (7/17)

AGCGGCCCAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATCTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTG  >  NC_000913/320925‑321073                                                                              |                                                                        aGCGGCCCAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATtttt                                                                      >  1:71651/1‑81 (MQ=255)   cGGCCCAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTc                                                                    <  1:71610/81‑1 (MQ=255)      cccAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTcaca                                                                 <  2:71611/81‑1 (MQ=255)      cccAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTcaca                                                                 <  2:71612/81‑1 (MQ=255)       ccAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACaa                                                                <  2:71613/81‑1 (MQ=255)        cAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAg                                                               <  1:71614/81‑1 (MQ=255)         aGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAgg                                                              >  2:71639/1‑81 (MQ=255)          gATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGc                                                             >  1:71644/1‑81 (MQ=255)                 tCCCGGGAATCGCTCGCTGTTGTGGTGATCGCTGGGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGt                                                       <  2:71615/80‑1 (MQ=255)                 tCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTc                                                      >  2:71642/1‑81 (MQ=255)                     gggAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCcgcg                                                  <  1:71616/81‑1 (MQ=255)                         aaCGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGtt                                              <  2:71617/81‑1 (MQ=255)                           cGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTg                                            >  2:71648/1‑81 (MQ=255)                                ccTGTTGTGGTGATCGCT‑‑GTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGt                                       <  2:71618/79‑1 (MQ=255)                                               gCTGAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAatat                        >  2:71646/1‑81 (MQ=255)                                                  gAGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCgg                     >  1:71654/1‑81 (MQ=255)                                                   aGTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCgg                     <  1:71619/80‑1 (MQ=255)                                                    gTCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGa                   <  2:71620/81‑1 (MQ=255)                                                     tCAGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGaa                  <  2:71621/81‑1 (MQ=255)                                                       aGTAGGCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAg                <  1:71622/81‑1 (MQ=255)                                                           ggCTATGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGt             <  2:71623/80‑1 (MQ=255)                                                                tGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGa         <  2:71624/79‑1 (MQ=255)                                                                tGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGAc        <  2:71625/80‑1 (MQ=255)                                                                tGCCAGTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACa       <  2:71626/81‑1 (MQ=255)                                                                     gTGAATCATTTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTg  >  2:71652/1‑81 (MQ=255)                                                                              |                                                                        AGCGGCCCAGATGTTTTCCCGGGAAACGCTCCCTGTTGTGGTGATCGCTGAGTCAGTAGGCTATGCCAGTGAATCATCTTTTCACAAGGCGTTTGTCCGCGAGTTTGGTTGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTG  >  NC_000913/320925‑321073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑