Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,994,908	C→T	intergenic (‑5/+29)	ygeK ← / ← ygeL	putative DNA‑binding transcriptional regulator YgeK/protein YgeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,994,908	0	C	T	100.0%	30.9 / NA	10	intergenic (‑5/+29)	ygeK/ygeL	putative DNA‑binding transcriptional regulator YgeK/protein YgeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

TGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGATC  >  NC_000913/2994835‑2994981                                                                          |                                                                          tGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTTCACCTg                                                                     >  2:543764/1‑80 (MQ=255)                    tGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAaaataaa                                                  <  2:543748/80‑1 (MQ=255)                                 tATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg                                       >  1:543769/1‑78 (MQ=255)                                            aCCCATTTTACGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTacac                         <  1:543749/81‑1 (MQ=255)                                             cccATTTTACGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACt                        >  2:543770/1‑81 (MQ=255)                                               cATTTTACGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTcc                      <  1:543750/81‑1 (MQ=255)                                                     aCGAGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCtat                 <  2:543751/80‑1 (MQ=255)                                                        aGTTCGGCCCCCATCATTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCt             <  1:543752/81‑1 (MQ=255)                                                                   catcatTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGATc  >  2:543773/1‑81 (MQ=255)                                                                   catcatTTCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTATTCAGATc  <  1:543753/81‑1 (MQ=255)                                                                          |                                                                          TGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGATC  >  NC_000913/2994835‑2994981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑