Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,006,208	G→A	intergenic (‑422/‑54)	ygeV ← / → ygeW	putative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,006,208	0	G	A	100.0%	101.7 / NA	29	intergenic (‑422/‑54)	ygeV/ygeW	putative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/11);  total (18/11)

TGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGA  >  NC_000913/3006136‑3006281                                                                         |                                                                          tGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTca                                                                   >  1:545982/1‑81 (MQ=255) tGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTc                                                                    <  2:545953/80‑1 (MQ=255)  gCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTca                                                                   >  2:545987/1‑80 (MQ=255)  gCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTca                                                                   >  1:545983/1‑80 (MQ=255)   cAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTcaca                                                                 <  1:545955/81‑1 (MQ=255)   cAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTcac                                                                  <  2:545954/80‑1 (MQ=255)          gcgGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGaaa                                                           >  1:545981/1‑80 (MQ=255)          gcgGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGaaa                                                           >  2:545986/1‑80 (MQ=255)                 aTTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCAc                                                   >  1:545979/1‑81 (MQ=255)                    cTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACttt                                                >  1:545978/1‑81 (MQ=255)                      ggCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGc                                              >  2:545976/1‑81 (MQ=255)                       gCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGc                                              <  2:545956/80‑1 (MQ=255)                           acTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTc                                         <  2:545957/81‑1 (MQ=255)                             ttATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCAt                                       >  1:545980/1‑81 (MQ=255)                                 tGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCa                                   >  2:545994/1‑81 (MQ=255)                                      gTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTAcc                              <  2:545959/81‑1 (MQ=255)                                      gTCCCAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTAc                               <  2:545958/80‑1 (MQ=255)                                        cccAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCgg                            >  2:545985/1‑81 (MQ=255)                                          cAGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGa                          >  1:545984/1‑81 (MQ=255)                                           aGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGAc                         >  2:545992/1‑81 (MQ=255)                                           aGGTATAGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGAc                         >  1:546069/1‑81 (MQ=255)                                                taGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTat                    <  1:545960/81‑1 (MQ=255)                                                 aGCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTat                    >  1:545991/1‑80 (MQ=255)                                                  gCTGTGAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTatg                   <  1:725984/80‑1 (MQ=255)                                                     gtgAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTatgatg                >  2:546034/1‑80 (MQ=255)                                                      tgAAAACACCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTatgatg                <  1:545961/79‑1 (MQ=255)                                                             acCAATCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGt        >  1:545990/1‑80 (MQ=255)                                                                  tCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAAt    >  2:545997/1‑79 (MQ=255)                                                                  tCACTTTAGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGa  <  2:545962/81‑1 (MQ=255)                                                                         |                                                                          TGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGA  >  NC_000913/3006136‑3006281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑