Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,915	C→T	H384H (CAC→CAT)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,915	0	C	T	100.0%	89.0 / NA	25	H384H (CAC→CAT)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

CGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACTT  >  NC_000913/3009837‑3009988                                                                               |                                                                          cGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCt                                                                         >  2:546817/1‑81 (MQ=255)  ggCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTg                                                                        <  2:546770/81‑1 (MQ=255)         cGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCt                                                                 >  2:546804/1‑81 (MQ=255)             gATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGc                                                             >  2:546849/1‑81 (MQ=255)              aTCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGcc                                                            >  2:546805/1‑81 (MQ=255)                 ggCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCcgcg                                                         <  1:546772/81‑1 (MQ=255)                 ggCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCcgcg                                                         <  2:546771/81‑1 (MQ=255)                  gCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGa                                                        >  2:546793/1‑81 (MQ=255)                       ggCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTa                                                    <  2:546773/80‑1 (MQ=255)                            gggTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTg                                               >  1:546803/1‑80 (MQ=255)                                aaaGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATc                                          >  1:546809/1‑81 (MQ=255)                                aaaGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATc                                          >  1:546810/1‑81 (MQ=255)                                  aGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATccc                                        <  1:546774/81‑1 (MQ=255)                                   gAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCg                                       <  2:546775/81‑1 (MQ=255)                                    aaCCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGt                                      >  2:546822/1‑81 (MQ=255)                                            gcgcATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgctgg                              >  1:546808/1‑81 (MQ=255)                                              gcATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgctggct                            >  2:546815/1‑81 (MQ=255)                                               cATGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAgctggctg                           >  2:546800/1‑81 (MQ=255)                                                 tGCACCTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGctggctgg                          >  1:546811/1‑80 (MQ=255)                                                     ccTAACGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACa                     <  1:546776/81‑1 (MQ=255)                                                         aCGAAAAAACCTGGAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGt                 <  2:546777/81‑1 (MQ=255)                                                                      gAAATCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCa     <  2:546778/80‑1 (MQ=255)                                                                       aaaTCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACt   <  2:546779/81‑1 (MQ=255)                                                                        aaTCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACtt  <  1:546780/81‑1 (MQ=255)                                                                        aaTCTCATCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACt   >  2:546828/1‑80 (MQ=255)                                                                               |                                                                          CGGCATTCCGGTGATCGGCTTTGGCCCGGGTAAAGAACCTGAAGCGCATGCACCTAACGAAAAAACCTGGAAATCTCACCTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACAGAGTAATTACTCTTCACTT  >  NC_000913/3009837‑3009988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑