Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,842	C→A	E466D (GAG→GAT)	yqeB ←	XdhC‑CoxI family protein YqeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,842	0	C	A	100.0%	162.2 / NA	45	E466D (GAG→GAT)	yqeB	XdhC‑CoxI family protein YqeB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/23);  total (22/23)

TAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGC  >  NC_000913/3012766‑3012919                                                                             |                                                                              tAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAc                                                                           >  1:547448/1‑81 (MQ=255)  aaaaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAc                                                                           >  2:547460/1‑80 (MQ=255)   aaaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAcc                                                                          >  1:547449/1‑80 (MQ=255)    aaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCaa                                                                        >  1:547454/1‑81 (MQ=255)         ccaaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAg                                                                   <  1:547394/81‑1 (MQ=255)          caaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAgg                                                                  >  2:547453/1‑81 (MQ=255)           aaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAgg                                                                  >  1:547457/1‑80 (MQ=255)              caCTGCCAGGCCGTCGTTCAACAAGCCACGCGCCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAAt                                                              <  2:547395/81‑1 (MQ=255)               aCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATc                                                             <  2:547396/81‑1 (MQ=255)                  gCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATcaca                                                          >  2:547458/1‑81 (MQ=255)                    cAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATc                                                        <  1:547397/81‑1 (MQ=255)                    cAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATc                                                        <  2:547398/81‑1 (MQ=255)                     aGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCg                                                       >  1:547451/1‑81 (MQ=255)                       gCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGcc                                                     <  1:547399/81‑1 (MQ=255)                       gCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGcc                                                     >  2:547455/1‑81 (MQ=255)                        ccGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGccc                                                    <  2:547400/81‑1 (MQ=255)                           tcgtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCctct                                                 >  2:547450/1‑81 (MQ=255)                             gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt                                               <  1:547402/81‑1 (MQ=255)                             gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt                                               >  1:547470/1‑81 (MQ=255)                             gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCtt                                                <  2:547401/80‑1 (MQ=255)                              ttCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt                                               >  1:547478/1‑80 (MQ=255)                                 aacaaGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                           <  1:547403/81‑1 (MQ=255)                                   caaGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAg                                         <  1:547404/81‑1 (MQ=255)                                      gCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATc                                      <  2:547405/81‑1 (MQ=255)                                            cACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTaa                                >  1:547452/1‑81 (MQ=255)                                             aCCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAt                               >  1:547474/1‑81 (MQ=255)                                              ccATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt                              <  1:547408/81‑1 (MQ=255)                                              ccATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt                              <  2:547407/81‑1 (MQ=255)                                              ccATCCCCGTCAACGGCGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt                              <  2:547406/81‑1 (MQ=255)                                               cATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt                             >  2:547463/1‑81 (MQ=255)                                               cATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt                             <  1:547409/81‑1 (MQ=255)                                                aTCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt                             >  2:547459/1‑80 (MQ=255)                                                 tCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCa                           >  2:547447/1‑81 (MQ=255)                                                   cccGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCat                          <  1:547411/80‑2 (MQ=255)                                                   cccGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCAc                          <  1:547410/80‑1 (MQ=255)                                                        cAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGa                    >  1:547491/1‑81 (MQ=255)                                                         aaCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt                   >  2:547468/1‑81 (MQ=255)                                                         aaCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt                   <  1:547412/81‑1 (MQ=255)                                                          aCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATc                  <  2:547413/81‑1 (MQ=255)                                                              tGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCa               >  2:547464/1‑80 (MQ=255)                                                               gCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCAt              >  1:547465/1‑80 (MQ=255)                                                                   tAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAAt           <  1:547414/79‑1 (MQ=255)                                                                    aaTTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGcc        >  2:547481/1‑81 (MQ=255)                                                                      tttCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTg      <  2:547415/81‑1 (MQ=255)                                                                          aTGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgcagc  <  1:547416/81‑1 (MQ=255)                                                                             |                                                                              TAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGC  >  NC_000913/3012766‑3012919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑