Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,071,721	G→A	A301V (GCG→GTG)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,071,721	0	G	A	100.0%	186.9 / NA	51	A301V (GCG→GTG)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/27);  total (24/27)

AGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACAT  >  NC_000913/3071645‑3071796                                                                             |                                                                            aGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACAtt                                                                         >  2:561248/1‑81 (MQ=255) aGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACAt                                                                          <  1:561201/80‑1 (MQ=255)  gCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACAtt                                                                         <  1:561202/80‑1 (MQ=255)   ccACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACAttct                                                                       <  2:561203/81‑1 (MQ=255)        aTTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAgg                                                                  <  2:561205/81‑1 (MQ=255)         tttCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGa                                                                 >  2:561262/1‑81 (MQ=255)           tCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGAtt                                                               >  1:561254/1‑81 (MQ=255)           tCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGAt                                                                <  1:561206/80‑1 (MQ=255)           tCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGAt                                                                <  1:561207/80‑1 (MQ=255)            cAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGAtt                                                               <  2:561208/80‑1 (MQ=255)             aGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGAttt                                                              <  2:561209/80‑1 (MQ=255)              gTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCa                                                            <  2:561210/81‑1 (MQ=255)                  cTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGc                                                          >  2:561255/1‑79 (MQ=255)                     tGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAg                                                       <  1:561211/79‑1 (MQ=255)                       cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTcc                                                   <  2:561212/81‑1 (MQ=255)                          aaGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCt                                                  >  2:561264/1‑79 (MQ=255)                            gTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGc                                              <  2:561213/81‑1 (MQ=255)                              tCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCg                                             >  1:561257/1‑80 (MQ=255)                               cGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCgg                                            >  1:561266/1‑80 (MQ=255)                                 ggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAg                                         <  2:561215/81‑1 (MQ=255)                                 ggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAg                                         <  1:561214/81‑1 (MQ=255)                                 ggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAg                                         >  1:561287/1‑81 (MQ=255)                                   aaTTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCa                                       >  1:561272/1‑81 (MQ=255)                                     ttCGAACACGCCAACCGGACCGTTCCAGAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATc                                     <  1:561216/81‑1 (MQ=255)                                     ttCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCAt                                      >  2:561281/1‑80 (MQ=255)                                       cGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCAc                                   >  2:561265/1‑81 (MQ=255)                                        gAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCAcc                                  >  2:561300/1‑81 (MQ=255)                                          acacGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGa                                <  1:561217/81‑1 (MQ=255)                                          acacGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGa                                >  1:561286/1‑81 (MQ=255)                                            acGCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGata                              <  1:561218/81‑1 (MQ=255)                                              gCCAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATc                            >  1:561275/1‑81 (MQ=255)                                               ccAACCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATcc                           <  2:561219/81‑1 (MQ=255)                                                  aCCGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAg                         >  1:561282/1‑80 (MQ=255)                                                   ccGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGa                       >  2:561290/1‑81 (MQ=255)                                                   ccGGACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGa                       <  2:561220/81‑1 (MQ=255)                                                     ggACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATc                     <  2:561221/81‑1 (MQ=255)                                                     ggACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATc                     >  2:561269/1‑81 (MQ=255)                                                      gACCGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCt                    <  1:561222/81‑1 (MQ=255)                                                         cGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCt                 <  2:561223/81‑1 (MQ=255)                                                         cGTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCt                 >  1:561278/1‑81 (MQ=255)                                                          gTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTc                <  2:561224/81‑1 (MQ=255)                                                          gTTCCACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTc                >  1:561280/1‑81 (MQ=255)                                                             ccACAGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGt              >  2:561267/1‑80 (MQ=255)                                                              cacaGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTc             <  2:561226/80‑1 (MQ=255)                                                              cacaGAATGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCa            <  2:561225/81‑1 (MQ=255)                                                                   aaTGGTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCttt       <  2:561227/81‑1 (MQ=255)                                                                      ggTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTca     >  2:561293/1‑80 (MQ=255)                                                                       gTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTcaca   >  1:561276/1‑81 (MQ=255)                                                                       gTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTcaca   >  1:561288/1‑81 (MQ=255)                                                                       gTTTTCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTcaca   >  1:561294/1‑81 (MQ=255)                                                                        ttttCACATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACAt  <  2:561228/81‑1 (MQ=255)                                                                             |                                                                            AGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACAT  >  NC_000913/3071645‑3071796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑