Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,994	G→A	R214R (CGC→CGT)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,994	0	G	A	100.0%	178.5 / NA	49	R214R (CGC→CGT)	tktA	transketolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/24);  total (25/24)

GGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCG  >  NC_000913/3080919‑3081069                                                                            |                                                                            ggAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAAt                                                                        <  2:563247/81‑1 (MQ=255)   aaGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATaa                                                                      >  2:563323/1‑81 (MQ=255)    aGGTTTGTCAGTCACTGCGCGCGCCTCCTCTACTGCGCGTCTGATAGATGCCGCGTCATGACCGTCGATCTCACGAATAAc                                                                     >  2:563309/1‑81 (MQ=255)         tGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGc                                                                 >  1:563300/1‑80 (MQ=255)          gtcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGcc                                                                <  2:563248/80‑1 (MQ=255)          gtcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGc                                                                 >  2:563332/1‑79 (MQ=255)           tcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCa                                                               <  1:563249/80‑1 (MQ=255)              gtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCg                                                           >  1:563292/1‑81 (MQ=255)               tcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCg                                                           >  2:563314/1‑80 (MQ=255)                caCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGt                                                          >  2:563326/1‑80 (MQ=255)                 aCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTa                                                         <  1:563250/80‑1 (MQ=255)                    gcgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCt                                                     >  1:563313/1‑81 (MQ=255)                     cgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCtt                                                    <  2:563252/81‑1 (MQ=255)                     cgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCtt                                                    <  2:563251/81‑1 (MQ=255)                      gcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTc                                                   >  1:563308/1‑81 (MQ=255)                             tcttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg                                             >  2:563334/1‑80 (MQ=255)                             tcttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg                                             >  1:563298/1‑80 (MQ=255)                              cttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACg                                             <  1:563253/79‑1 (MQ=255)                               ttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAt                                          >  2:563329/1‑81 (MQ=255)                                 ctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAt                                          <  1:563254/79‑1 (MQ=255)                                   aCTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg                                      >  2:563302/1‑81 (MQ=255)                                    cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGc                                       <  1:563255/79‑1 (MQ=255)                                    cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg                                      <  1:563256/80‑1 (MQ=255)                                     tGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt                                    >  1:563304/1‑81 (MQ=255)                                      gcgcGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtg                                   >  1:563343/1‑81 (MQ=255)                                      gcgcGTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtg                                   >  1:563312/1‑81 (MQ=255)                                        gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtc                                 <  1:563258/81‑1 (MQ=255)                                        gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtc                                 <  1:563257/81‑1 (MQ=255)                                          gTTTGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcgt                               >  2:563327/1‑81 (MQ=255)                                            ttGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGtcgtcg                             >  2:563311/1‑81 (MQ=255)                                             tGATAGATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGtcgtcg                             <  2:563260/80‑1 (MQ=255)                                                  gATGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc                       <  1:563261/81‑1 (MQ=255)                                                   aTGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAcc                      <  2:563262/81‑1 (MQ=255)                                                    tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAc                       >  1:563320/1‑79 (MQ=255)                                                    tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCa                     <  2:563264/81‑1 (MQ=255)                                                    tGCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCa                     <  1:563263/81‑1 (MQ=255)                                                     gCCGCGTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAATCAg                    >  1:563331/1‑81 (MQ=255)                                                        gcgTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCt                 >  2:563324/1‑81 (MQ=255)                                                         cgTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt                <  2:563265/81‑1 (MQ=255)                                                          gTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt                >  2:563316/1‑80 (MQ=255)                                                          gTCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCtt                >  2:563328/1‑80 (MQ=255)                                                           tCATGACCGTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCa              <  2:563266/81‑1 (MQ=255)                                                                   gTCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGAc       >  1:563305/1‑80 (MQ=255)                                                                    tCGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCa     <  2:563267/81‑1 (MQ=255)                                                                     cGATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCAt    >  2:563330/1‑81 (MQ=255)                                                                      gATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCAt    <  1:563268/80‑1 (MQ=255)                                                                      gATGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATc   <  2:563269/81‑1 (MQ=255)                                                                       aTGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCg  <  2:563270/81‑1 (MQ=255)                                                                       aTGTCACGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCg  <  1:563271/81‑1 (MQ=255)                                                                            |                                                                            GGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCG  >  NC_000913/3080919‑3081069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑