Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,719	T→A	D337V (GAT→GTT)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,719	0	T	A	100.0%	140.3 / NA	40	D337V (GAT→GTT)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/16);  total (24/16)

CTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCC  >  NC_000913/3099641‑3099795                                                                               |                                                                             cTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACt                                                                            >  2:567818/1‑81 (MQ=255)    aaGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACttt                                                                          <  2:567764/80‑1 (MQ=255)      gTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGt                                                                       <  1:567765/81‑1 (MQ=255)      gTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGt                                                                       >  1:567791/1‑81 (MQ=255)            aaGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGt                                                                 >  1:567809/1‑81 (MQ=255)              gCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCa                                                               <  1:567766/81‑1 (MQ=255)                 ccGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCagag                                                            <  2:567768/81‑1 (MQ=255)                 ccGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCagag                                                            <  1:567767/81‑1 (MQ=255)                  cGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGc                                                           <  1:567769/81‑1 (MQ=255)                   ggCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGc                                                           >  1:567812/1‑80 (MQ=255)                       cGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGtt                                                      <  1:567770/81‑1 (MQ=255)                         aTACCGGGGCGCGGCGATTAGTACTGATTGAAGATCCGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGttt                                                     >  1:567813/1‑79 (MQ=255)                         aTACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTgc                                                    <  2:567771/81‑1 (MQ=255)                              ggggCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTgcag                                                  >  1:567807/1‑78 (MQ=255)                                  cgaagcgaTTAGTACTGATTGTAGATCTGCTGGATCTGCTGCGGAACTTTGATTTGCGTCAGAACCAGTTGCAGCAGAAc                                            <  2:567772/80‑1 (MQ=255)                                   gaggcgaTTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcgc                                          >  1:567819/1‑81 (MQ=255)                                        gaTTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCt                                     >  2:567808/1‑81 (MQ=255)                                          ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt                                   >  1:567810/1‑81 (MQ=255)                                          ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt                                   >  1:567820/1‑81 (MQ=255)                                          ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt                                   <  1:567773/81‑1 (MQ=255)                                            aGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttttt                                 >  1:567800/1‑81 (MQ=255)                                               aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg                              >  2:567805/1‑81 (MQ=255)                                               aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg                              >  1:567823/1‑81 (MQ=255)                                               aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg                              >  1:567816/1‑81 (MQ=255)                                                   aTTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGtt                          >  2:567815/1‑81 (MQ=255)                                                      gAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTCCGGGTTCAg                       >  2:567814/1‑81 (MQ=255)                                                        aGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCg                     >  2:567826/1‑81 (MQ=255)                                                        aGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCg                     >  2:567811/1‑81 (MQ=255)                                                         gatctgctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGt                    <  1:567774/81‑1 (MQ=255)                                                              gctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGc                  >  2:567829/1‑78 (MQ=255)                                                               ctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCag               <  1:567775/80‑1 (MQ=255)                                                                 ggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAgg            >  2:567806/1‑81 (MQ=255)                                                                     ctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgac        >  1:567840/1‑81 (MQ=255)                                                                       gctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacg       >  2:567802/1‑80 (MQ=255)                                                                       gctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacg       >  2:567817/1‑80 (MQ=255)                                                                        ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacga      <  2:567777/80‑1 (MQ=255)                                                                        ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgac        <  1:567778/78‑1 (MQ=255)                                                                        ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcg          <  1:567776/76‑1 (MQ=255)                                                                           cGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGcc  <  2:567779/81‑1 (MQ=255)                                                                           cGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGcc  >  2:567838/1‑81 (MQ=255)                                                                               |                                                                             CTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCC  >  NC_000913/3099641‑3099795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑