Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,141,969	G→C	P341A (CCG→GCG)	hybC ←	hydrogenase 2 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,141,969	0	G	C	100.0%	159.4 / NA	44	P341A (CCG→GCG)	hybC	hydrogenase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/23);  total (21/23)

CACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGA  >  NC_000913/3141895‑3142046                                                                           |                                                                              cACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCtt                                                                          <  2:577540/80‑1 (MQ=255)    ccAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCttcttc                                                                      >  2:577591/1‑81 (MQ=255)     cAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCt                                                                     <  2:577541/81‑1 (MQ=255)      aGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCtt                                                                    <  1:577542/81‑1 (MQ=255)       ggAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCttt                                                                   <  1:577543/81‑1 (MQ=255)       ggAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCttt                                                                   <  2:577544/81‑1 (MQ=255)          atatTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTtata                                                                <  1:577545/81‑1 (MQ=255)            atTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATAc                                                               >  2:577593/1‑80 (MQ=255)             tttCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATAc                                                               >  1:577600/1‑79 (MQ=255)               tCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAg                                                            >  2:577596/1‑80 (MQ=255)                cccGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAg                                                            >  2:577595/1‑79 (MQ=255)                   gtcgtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTg                                                       <  1:577546/81‑1 (MQ=255)                   gtcgtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTg                                                       >  1:577606/1‑81 (MQ=255)                      gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCtt                                                    <  1:577548/81‑1 (MQ=255)                      gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCtt                                                    >  2:577597/1‑81 (MQ=255)                      gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCtt                                                    >  1:577604/1‑81 (MQ=255)                      gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCt                                                     <  1:577547/80‑1 (MQ=255)                       tcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTc                                                   <  2:577549/81‑1 (MQ=255)                         aGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTcgc                                                 <  1:577550/81‑1 (MQ=255)                         aGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTcgc                                                 <  1:577551/81‑1 (MQ=255)                           accaaccaTCATAAGTTGGAATGGTGGTGCATTCCCACGGTGCATGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCgcgc                                               >  2:953915/1‑81 (MQ=255)                           accaaccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCgcgc                                               >  1:577612/1‑81 (MQ=255)                           accaaccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCgcgc                                               >  2:577611/1‑81 (MQ=255)                            ccaaccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCt                                              >  2:577602/1‑81 (MQ=255)                             caaccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCtt                                             >  2:577607/1‑81 (MQ=255)                             caaccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCtt                                             >  1:577617/1‑81 (MQ=255)                                  atcatAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCt                                         >  2:577613/1‑80 (MQ=255)                                     atAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGaa                                      <  1:577552/80‑1 (MQ=255)                                        aGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGcc                                  <  1:577554/81‑1 (MQ=255)                                        aGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGc                                   <  1:577553/80‑1 (MQ=255)                                          ccGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCtt                                <  2:577555/81‑1 (MQ=255)                                           cGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCttt                               >  1:577598/1‑81 (MQ=255)                                           cGGAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCttt                               <  1:577556/81‑1 (MQ=255)                                            ggAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg                              <  1:577558/81‑1 (MQ=255)                                            ggAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg                              <  2:577557/81‑1 (MQ=255)                                             gAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGa                             >  1:577605/1‑81 (MQ=255)                                             gAATGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGa                             >  2:577623/1‑81 (MQ=255)                                               aTGGTGGTGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGAt                            >  2:577610/1‑80 (MQ=255)                                                 ggtggtGCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAg                         >  1:577603/1‑81 (MQ=255)                                                      tgCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTAtt                    <  1:577559/81‑1 (MQ=255)                                                      tgCCTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTAtt                    <  2:577560/81‑1 (MQ=255)                                                        ccTTCCCACGGTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTc                   <  2:577561/80‑1 (MQ=255)                                                                 ggTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAAt           >  1:577618/1‑79 (MQ=255)                                                                  gTGCCTGCGCCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATgag        <  2:577562/81‑1 (MQ=255)                                                                        gcgccgCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGa  >  2:577630/1‑81 (MQ=255)                                                                           |                                                                              CACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTATTCATCGGAATGAGAAGTGA  >  NC_000913/3141895‑3142046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑