Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	331,597	G→A	intergenic (+101/‑205)	betT → / → yahV	choline:H(+) symporter/protein YahV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	331,597	0	G	A	100.0%	87.1 / NA	25	intergenic (+101/‑205)	betT/yahV	choline:H(+) symporter/protein YahV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/15);  total (10/15)

GTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATG  >  NC_000913/331522‑331671                                                                            |                                                                           gTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTAc                                                                       >  2:73819/1‑81 (MQ=255)  tCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTAc                                                                       >  2:73820/1‑80 (MQ=255)       atatGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGac                                                                  >  2:73813/1‑80 (MQ=255)          tGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGacaa                                                                >  2:73822/1‑79 (MQ=255)             cgcTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGat                                                           <  1:73781/81‑1 (MQ=255)              gcTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGata                                                          <  2:73782/81‑1 (MQ=255)                     ctcCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATaaaa                                                    <  2:73783/80‑1 (MQ=255)                       ccTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATaaaa                                                    >  1:73816/1‑78 (MQ=255)                        cTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAaaaaga                                                <  1:73785/81‑1 (MQ=255)                        cTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAaaaaga                                                <  2:73784/81‑1 (MQ=255)                              ctTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAaagaaaagaa                                          >  2:73823/1‑81 (MQ=255)                                 gATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTa                                       >  1:73797/1‑81 (MQ=255)                                   tACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTAtt                                     <  1:73786/81‑1 (MQ=255)                                      gTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTg                                    >  1:73825/1‑79 (MQ=255)                                         atatCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGc                                <  1:73787/80‑1 (MQ=255)                                          tatCTATACGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGcc                               <  1:73788/80‑1 (MQ=255)                                                taCGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCaaaa                          <  1:73789/79‑1 (MQ=255)                                                taCGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCaa                            <  1:73790/77‑1 (MQ=255)                                                  cGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCt                       <  2:73792/80‑1 (MQ=255)                                                  cGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCt                       <  2:73793/80‑1 (MQ=255)                                                  cGGTTAAGCCCTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTa                      <  2:73791/81‑1 (MQ=255)                                                            cTTAGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCt            <  2:73794/81‑1 (MQ=255)                                                               aGTATCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGtt         >  2:73827/1‑81 (MQ=255)                                                                  aTCTATTGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGc       >  1:73830/1‑80 (MQ=255)                                                                       ttGATAATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATg  <  1:73795/80‑1 (MQ=255)                                                                            |                                                                           GTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATG  >  NC_000913/331522‑331671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑