Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,531	G→A	L139L (TTG→TTA)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,531	0	G	A	100.0%	150.8 / NA	41	L139L (TTG→TTA)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

CAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAC  >  NC_000913/3178460‑3178607                                                                        |                                                                             cAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAt                                                                      <  2:586370/80‑1 (MQ=255)  aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAtt                                                                     >  2:586422/1‑80 (MQ=255)  aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTAtt                                                                     >  2:586434/1‑80 (MQ=255)  aGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTg                                                                    <  2:586371/81‑1 (MQ=255)   ggACGTCACGTATCAGACCGATCAGCAAACATTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGa                                                                   <  1:586372/81‑1 (MQ=255)      cGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGt                                                                <  1:586373/81‑1 (MQ=255)      cGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGt                                                                <  1:586374/81‑1 (MQ=255)       gTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGtt                                                               <  1:586375/81‑1 (MQ=255)        tCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTc                                                              <  2:586376/81‑1 (MQ=255)          aCGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCtt                                                            <  1:586377/81‑1 (MQ=255)          aCGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCtt                                                            >  2:586420/1‑81 (MQ=255)            gTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTc                                                          >  1:586424/1‑81 (MQ=255)             tATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTcc                                                         <  1:586378/81‑1 (MQ=255)             tATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTcc                                                         <  2:586379/81‑1 (MQ=255)                 aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtata                                                     >  1:586423/1‑81 (MQ=255)                 aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat                                                      <  2:586382/80‑1 (MQ=255)                 aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat                                                      <  2:586381/80‑1 (MQ=255)                 aGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCtat                                                      <  1:586380/80‑1 (MQ=255)                   aCCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATaca                                                   >  1:586429/1‑81 (MQ=255)                        tCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAgg                                               >  2:586448/1‑80 (MQ=255)                             aaaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaa                                          >  2:586451/1‑80 (MQ=255)                             aaaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaa                                          >  2:586442/1‑80 (MQ=255)                              aaCCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACaaa                                         >  2:586428/1‑80 (MQ=255)                                  ttGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAaag                                      >  2:586427/1‑79 (MQ=255)                                    gATCCTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGc                                  >  1:586437/1‑81 (MQ=255)                                        cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCGTATACACAGGCACAAAAAGAAGCGATc                              >  2:586449/1‑81 (MQ=255)                                        cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATc                              <  2:586383/81‑1 (MQ=255)                                        cTCAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATc                              <  2:586384/81‑1 (MQ=255)                                          cAACACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTa                            >  1:586447/1‑81 (MQ=255)                                           aaCACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCt                             <  1:586385/79‑1 (MQ=255)                                           aaCACCGCGACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTAc                           >  2:586433/1‑81 (MQ=255)                                                cgcgACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCa                      <  2:586386/81‑1 (MQ=255)                                                   gACCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCATCAAt                    >  2:586436/1‑80 (MQ=255)                                                    aCCGCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTa                  <  1:586387/81‑1 (MQ=255)                                                       gCTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAt               <  1:586388/81‑1 (MQ=255)                                                        cTTATTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAt               >  1:586443/1‑80 (MQ=255)                                                           aTTTCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCaaa           >  2:586445/1‑81 (MQ=255)                                                            tttCAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAac          >  2:586446/1‑81 (MQ=255)                                                               cAACGTGTTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAacca        <  2:586389/80‑1 (MQ=255)                                                                    tgtTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGTTCTACCGTCAATTAGATCAAACCACCCAAc  <  2:586391/81‑1 (MQ=255)                                                                    tgtTAAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAc  <  2:586390/81‑1 (MQ=255)                                                                        |                                                                             CAGGACGTCACGTATCAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAATGCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGATCAAACCACCCAAC  >  NC_000913/3178460‑3178607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑