Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,192,830	T→C	I208T (ATA→ACA)	yqiJ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,192,830	0	T	C	100.0%	61.7 / NA	19	I208T (ATA→ACA)	yqiJ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/5);  total (14/5)

ACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGAT  >  NC_000913/3192751‑3192898                                                                                |                                                                     aCTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAata                                                                     >  1:589053/1‑81 (MQ=255)           aCAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATtta                                                           <  2:589039/81‑1 (MQ=255)               aGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTaa                                                       >  2:589062/1‑81 (MQ=255)                  gtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATc                                                     >  2:589060/1‑80 (MQ=255)                     aaTTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCaa                                                   >  2:589059/1‑79 (MQ=255)                             gCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAAtt                                          >  1:589054/1‑80 (MQ=255)                                gACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTtaat                                      <  2:589040/81‑1 (MQ=255)                                 aCTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTtaat                                      >  1:589063/1‑80 (MQ=255)                                    ttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                  >  2:589058/1‑81 (MQ=255)                                      cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                 <  2:589041/80‑1 (MQ=255)                                      cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGga                                >  1:589070/1‑81 (MQ=255)                                          aaCGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatga                             >  2:589057/1‑80 (MQ=255)                                           aCGCGATATTTGGCGGAAAATAATCCCTGGCCCTAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGata                           >  1:589067/1‑81 (MQ=255)                                               gATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                       <  2:589042/81‑1 (MQ=255)                                               gATATTTGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                       >  1:589056/1‑81 (MQ=255)                                                   tttGGCGGAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAt                   <  1:589043/81‑1 (MQ=255)                                                         ggAAAATAATCCCTGGTCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCtgtg             >  2:589100/1‑81 (MQ=255)                                                          gAAAATAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGc            >  1:589071/1‑81 (MQ=255)                                                            aaaTAATCCCTGGCCCCAAACATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGccc          >  2:589102/1‑81 (MQ=255)                                                                    ccTGGTCCCAAACTTTGTAATTTATTAATCAAAAGGAAATCTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGAt  >  2:589073/1‑81 (MQ=255)                                                                                |                                                                     ACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGAT  >  NC_000913/3192751‑3192898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑