Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,798	C→T	G319D (GGC→GAC)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,798	0	C	T	100.0%	206.0 / NA	56	G319D (GGC→GAC)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

GTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGCCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCAC  >  NC_000913/3195720‑3195874                                                                               |                                                                             gTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCt                                                                            <  2:589689/81‑1 (MQ=255) gTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCt                                                                            <  2:589688/81‑1 (MQ=255)    atcaCCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGt                                                                          <  1:589690/80‑1 (MQ=255)       accacTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCt                                                                      <  1:589691/81‑1 (MQ=255)       accacTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCt                                                                      >  2:589764/1‑81 (MQ=255)           cTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCAc                                                                  <  2:589692/81‑1 (MQ=255)           cTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCAc                                                                  >  1:589775/1‑81 (MQ=255)              tttCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGt                                                                <  1:589693/80‑1 (MQ=255)               ttCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTc                                                               >  2:589786/1‑80 (MQ=255)                tCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTcc                                                              <  1:589694/80‑1 (MQ=255)                tCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTcc                                                              <  1:589695/80‑1 (MQ=255)                  accacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCAc                                                            <  1:589696/80‑1 (MQ=255)                   ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACg                                                           >  2:589757/1‑80 (MQ=255)                   ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACg                                                           >  1:589720/1‑80 (MQ=255)                   ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGt                                                          <  2:589697/81‑1 (MQ=255)                    cacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa                                                         >  2:589793/1‑81 (MQ=255)                    cacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa                                                         >  2:589746/1‑81 (MQ=255)                     acGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa                                                         >  1:589774/1‑80 (MQ=255)                        tttACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGc                                                     <  2:589698/81‑1 (MQ=255)                         ttACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCa                                                    >  1:589769/1‑81 (MQ=255)                            cgcgCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCAtt                                                  <  1:589699/80‑1 (MQ=255)                               gcTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTc                                               >  1:589782/1‑80 (MQ=255)                                cTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCa                                             <  2:589700/81‑1 (MQ=255)                                   ccGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAg                                            <  2:589701/79‑1 (MQ=255)                                    cGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTc                                         <  1:589703/81‑1 (MQ=255)                                    cGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTc                                         <  2:589702/81‑1 (MQ=255)                                     gCTACGGCCAGCTTCAGTTCCTCTTTGGTCATCACGCCAAAGTCTGTATCCGCACGTCCACGTATAGCATTTTCCAGCTc                                         >  1:589771/1‑80 (MQ=255)                                      cTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGa                                       <  1:589705/81‑1 (MQ=255)                                      cTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGa                                       <  2:589704/81‑1 (MQ=255)                                       tACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGAt                                      >  1:589763/1‑81 (MQ=255)                                         cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGAt                                      <  1:589708/79‑1 (MQ=255)                                         cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATc                                     <  1:589707/80‑1 (MQ=255)                                         cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCg                                    <  1:589706/81‑1 (MQ=255)                                               gCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGaaa                              >  2:589787/1‑81 (MQ=255)                                               gCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGaaa                              >  2:589784/1‑81 (MQ=255)                                                cTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAAc                             <  2:589709/81‑1 (MQ=255)                                                  tCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCATGTCCACGCACAGCATTTTCCAGCTCGATCGGCGAAACCg                           >  2:589762/1‑81 (MQ=255)                                                    aGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTg                         >  2:589792/1‑81 (MQ=255)                                                    aGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTg                         >  2:589785/1‑81 (MQ=255)                                                           ctTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc                  >  2:589783/1‑81 (MQ=255)                                                           ctTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc                  >  2:589772/1‑81 (MQ=255)                                                              cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTccc                <  1:589711/80‑1 (MQ=255)                                                              cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTcc                 >  2:589778/1‑79 (MQ=255)                                                              cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc                  <  1:589710/78‑1 (MQ=255)                                                               ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCa               <  1:589712/80‑1 (MQ=255)                                                               ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAg              >  1:589768/1‑81 (MQ=255)                                                               ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAg              >  1:589770/1‑81 (MQ=255)                                                                gTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGt             >  2:589761/1‑81 (MQ=255)                                                                   atcaCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTg          >  2:589790/1‑81 (MQ=255)                                                                     caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc        >  2:589779/1‑81 (MQ=255)                                                                     caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc        >  2:589760/1‑81 (MQ=255)                                                                     caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc        >  1:589789/1‑81 (MQ=255)                                                                      aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc        <  1:589715/80‑1 (MQ=255)                                                                      aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCg       <  2:589713/81‑1 (MQ=255)                                                                      aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCg       <  1:589714/81‑1 (MQ=255)                                                                           aaaGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGaccac  >  2:589781/1‑81 (MQ=255)                                                                               |                                                                             GTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGCCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCAC  >  NC_000913/3195720‑3195874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑