Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,047	A→T	Y90N (TAT→AAT)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,047	0	A	T	100.0%	91.6 / NA	26	Y90N (TAT→AAT)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/11);  total (15/11)

CGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242969‑3243123                                                                               |                                                                             cGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTCGATAGCGATTCt                                                                            >  2:601394/1‑81 (MQ=255)   ttAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGa                                                                          >  2:601402/1‑81 (MQ=255)        gcgACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAg                                                                     <  2:601354/81‑1 (MQ=255)         cgacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGa                                                                    <  1:601355/81‑1 (MQ=255)             gaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGc                                                                >  1:601417/1‑81 (MQ=255)                 gATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCa                                                             <  2:601356/80‑1 (MQ=255)                    cTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGAtt                                                         >  2:601404/1‑81 (MQ=255)                       cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGAtt                                                         <  2:601357/78‑1 (MQ=255)                        aCTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTg                                                     >  1:601403/1‑81 (MQ=255)                                   tGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtgtg                                          >  1:601413/1‑81 (MQ=255)                                      cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc                                       >  1:601398/1‑81 (MQ=255)                                      cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc                                       >  2:601399/1‑81 (MQ=255)                                        cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt                                     >  2:601414/1‑81 (MQ=255)                                              caggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               <  1:601358/81‑1 (MQ=255)                                              caggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                               >  1:601406/1‑81 (MQ=255)                                                  cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCgtgaacgtgaac                           <  1:601359/81‑1 (MQ=255)                                                    gATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAacac                         >  2:601407/1‑81 (MQ=255)                                                      tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt                       <  2:601360/81‑1 (MQ=255)                                                        cTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGc                     <  2:601361/81‑1 (MQ=255)                                                           aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg                   >  2:601415/1‑80 (MQ=255)                                                                cAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAatat             >  1:601400/1‑81 (MQ=255)                                                                  ggTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATcc           >  2:601401/1‑81 (MQ=255)                                                                    ttGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGc         <  1:601362/81‑1 (MQ=255)                                                                      gATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGcc        <  1:601363/80‑1 (MQ=255)                                                                          gCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATg    <  2:601364/80‑1 (MQ=255)                                                                            gATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCa  >  1:601410/1‑80 (MQ=255)                                                                               |                                                                             CGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGCCAATGCA  >  NC_000913/3242969‑3243123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑