Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,255,204	G→A	A54A (GCG→GCA)	yhaL →	uncharacterized protein YhaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,255,204	0	G	A	100.0%	120.6 / NA	33	A54A (GCG→GCA)	yhaL	uncharacterized protein YhaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

AAGAGATGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCGTAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTAAACAA  >  NC_000913/3255131‑3255280                                                                          |                                                                             aaGAGATGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGa                                                                        >  2:604117/1‑80 (MQ=255) aaGAGATGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGAc                                                                       <  1:604069/81‑1 (MQ=255)     gaTGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGt                                                                   <  1:604070/81‑1 (MQ=255)       tGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTg                                                                 <  2:604072/81‑1 (MQ=255)       tGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTg                                                                 <  2:604071/81‑1 (MQ=255)        gTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGc                                                                >  2:604110/1‑81 (MQ=255)         ttGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGcc                                                               <  1:604073/81‑1 (MQ=255)            agcgagcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGa                                                            >  2:604125/1‑81 (MQ=255)             gcgagcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGAt                                                           <  2:604074/81‑1 (MQ=255)               gagcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgc                                                         <  1:604075/81‑1 (MQ=255)                agcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcg                                                        >  2:604116/1‑81 (MQ=255)                agcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcg                                                        >  1:604115/1‑81 (MQ=255)                agcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcg                                                        >  1:604112/1‑81 (MQ=255)                 gcTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcgg                                                       <  1:604076/81‑1 (MQ=255)                   tGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcggc                                                      >  2:604114/1‑80 (MQ=255)                     gAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATgcggcg                                                     <  2:604077/79‑1 (MQ=255)                        gCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAAc                                                >  1:604108/1‑81 (MQ=255)                                 gcgGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATcc                                       <  2:604078/81‑1 (MQ=255)                                  cgGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCg                                      <  1:604079/81‑1 (MQ=255)                                           aaaCGCGTTTAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACaaa                             <  2:604080/81‑1 (MQ=255)                                                  tttAGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCcaa                       >  1:604131/1‑80 (MQ=255)                                                     aGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCcaacaa                    >  2:604120/1‑80 (MQ=255)                                                     aGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCcaacaa                    >  1:604133/1‑80 (MQ=255)                                                     aGCCGAGGATGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCcaacaa                    >  2:604124/1‑80 (MQ=255)                                                             aTGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACg            >  1:604129/1‑80 (MQ=255)                                                              tGAAGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGaa          <  1:604081/81‑1 (MQ=255)                                                                aaGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAAt         >  1:604126/1‑80 (MQ=255)                                                                 aGCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAAtt        >  2:604128/1‑80 (MQ=255)                                                                  gCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTa       >  1:604119/1‑80 (MQ=255)                                                                  gCTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTa       >  2:604137/1‑80 (MQ=255)                                                                   cTACCGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTaaa     <  1:604082/81‑1 (MQ=255)                                                                      ccGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTAatcaa  <  2:604083/81‑1 (MQ=255)                                                                      ccGCATAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTAaacaa  >  1:604123/1‑81 (MQ=255)                                                                          |                                                                             AAGAGATGTTGAGCGAGCTGGAAGCTATCGTCGCGGATGCTGAAACGCGTTTAGCCGAGGATGAAGCTACCGCGTAAAGACAGGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAATCCAACAACATTAACGAATTAAACAA  >  NC_000913/3255131‑3255280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑