Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,324,530	C→T	A128T (GCG→ACG)	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,324,530	0	C	T	100.0%	123.0 / NA	34	A128T (GCG→ACG)	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/11);  total (23/11)

ACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATG  >  NC_000913/3324452‑3324606                                                                               |                                                                             aCTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCt                                                                            >  2:619996/1‑81 (MQ=255)  cTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGtctc                                                                           <  2:619957/81‑1 (MQ=255)     cGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTcc                                                                          <  1:619958/79‑1 (MQ=255)      ggAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCa                                                                         >  2:620004/1‑79 (MQ=255)          cTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAgcgc                                                                   >  2:620005/1‑81 (MQ=255)             ccTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCa                                                                 <  2:619959/80‑1 (MQ=255)                gCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGtt                                                             >  2:620012/1‑81 (MQ=255)                gCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGtt                                                             >  1:620014/1‑81 (MQ=255)                     gTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGaa                                                        <  2:619960/81‑1 (MQ=255)                      tttttGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGa                                                         >  1:620000/1‑79 (MQ=255)                          tGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAgg                                                     >  2:619995/1‑79 (MQ=255)                              tttCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCg                                                >  2:620006/1‑80 (MQ=255)                               ttCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGa                                              >  1:620024/1‑81 (MQ=255)                                tCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGa                                              <  2:619961/80‑1 (MQ=255)                                     gCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                                        >  2:619972/1‑81 (MQ=255)                                       atatGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAtt                                       >  2:619998/1‑80 (MQ=255)                                          tGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaat                                    <  1:619962/80‑1 (MQ=255)                                           gCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                                  >  1:620015/1‑81 (MQ=255)                                            cATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTaataat                                 >  1:620026/1‑81 (MQ=255)                                             aTCAGACAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtg                                >  2:620008/1‑81 (MQ=255)                                                  aCAAACCGGTAAACCGGTTTCTGCAGCCGTCACCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAgc                           <  2:619963/81‑1 (MQ=255)                                                   cAAACCGGTAAACCGGTTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAgcg                          <  1:619964/81‑1 (MQ=255)                                                    aaaccggtaaaccggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAgcgc                         >  1:620022/1‑81 (MQ=255)                                                      accggtaaaccggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGcc                        >  1:620011/1‑80 (MQ=255)                                                      accggtaaaccggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCa                       <  2:619965/81‑1 (MQ=255)                                                          gtaaaccggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACtt                   <  1:619966/81‑1 (MQ=255)                                                              accggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGc               >  1:620030/1‑81 (MQ=255)                                                              accggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGc               >  2:620013/1‑81 (MQ=255)                                                                 ggtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGa            >  1:620028/1‑81 (MQ=255)                                                                  gtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGAc           >  1:620018/1‑81 (MQ=255)                                                                  gtTTCTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGACAACTTTCGCTGAc           >  1:620023/1‑81 (MQ=255)                                                                      cTGCAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCAc       >  2:620019/1‑81 (MQ=255)                                                                         cAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACg      <  1:619967/79‑1 (MQ=255)                                                                         cAGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGa    >  2:620033/1‑81 (MQ=255)                                                                          aGCCGTCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGAt   <  1:619968/81‑1 (MQ=255)                                                                           gccgtcTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATg  <  1:619969/81‑1 (MQ=255)                                                                               |                                                                             ACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATG  >  NC_000913/3324452‑3324606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑