Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,334,826	A→C	intergenic (+145/‑83)	ispB → / → sfsB	all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,334,826	0	A	C	100.0%	79.1 / NA	23	intergenic (+145/‑83)	ispB/sfsB	all‑trans‑octaprenyl‑diphosphate synthase/putative transcriptional regulator SfsB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/9);  total (14/9)

TAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAG  >  NC_000913/3334754‑3334896                                                                         |                                                                       taACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAAt                                                                   <  1:622341/78‑1 (MQ=255)      ccTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATc                                                           <  2:622342/81‑1 (MQ=255)          aaaaaTAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAgg                                                        <  2:622343/80‑1 (MQ=255)            aaaTAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAg                                                      >  1:622371/1‑80 (MQ=255)            aaaTAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGt                                                     <  2:622344/81‑1 (MQ=255)                    aTGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGAt                                             >  2:622375/1‑81 (MQ=255)                    aTGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGAt                                             >  1:622369/1‑81 (MQ=255)                        gCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTc                                         >  1:622365/1‑81 (MQ=255)                              ttATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACag                                    >  2:622376/1‑80 (MQ=255)                              ttATATATTCTGAATATTCACACTCTTTACAGGAACTCTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACag                                    >  1:622366/1‑80 (MQ=255)                               tatataTTCTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGAc                                      <  2:622345/77‑1 (MQ=255)                                       cTGAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGtt                          >  2:622368/1‑81 (MQ=255)                                         gAATATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCt                        >  1:622359/1‑81 (MQ=255)                                          aaTATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGtt                          <  2:622346/78‑1 (MQ=255)                                          aaTATTCACACTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCt                        >  1:622377/1‑80 (MQ=255)                                                cacaCTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCtgaatga                  >  2:622367/1‑80 (MQ=255)                                                cacaCTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCtgaatga                  >  2:622373/1‑80 (MQ=255)                                                 acacTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCtgaatgaa                 <  1:622347/80‑1 (MQ=255)                                                   acTCTTTACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGc              >  2:622372/1‑81 (MQ=255)                                                         tACAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACt          <  1:622348/79‑1 (MQ=255)                                                           cAGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACt      >  1:622370/1‑81 (MQ=255)                                                            aGGAACTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACtt     <  2:622349/81‑1 (MQ=255)                                                                 cTTTTTAGCGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAg  >  1:622386/1‑79 (MQ=255)                                                                         |                                                                       TAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAG  >  NC_000913/3334754‑3334896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑