Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,040	A→C	V9G (GTC→GGC)	mlaF ←	intermembrane phospholipid transport system, ATP binding subunit MlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,040	0	A	C	100.0%	152.5 / NA	43	V9G (GTC→GGC)	mlaF	intermembrane phospholipid transport system, ATP binding subunit MlaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/26);  total (17/26)

CCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAC  >  NC_000913/3339964‑3340116                                                                             |                                                                             cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt                                                                            <  1:623603/79‑1 (MQ=255) cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt                                                                            <  1:623602/79‑1 (MQ=255) cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt                                                                            <  2:623604/79‑1 (MQ=255) cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTa                                                                           <  2:623605/80‑1 (MQ=255) cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTa                                                                           <  2:623601/80‑1 (MQ=255)    tCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAAtt                                                                       >  2:623668/1‑81 (MQ=255)      gcgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTc                                                                      <  2:623606/80‑1 (MQ=255)        ggCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGc                                                                    >  1:623664/1‑80 (MQ=255)         gCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCca                                                                  >  2:623691/1‑81 (MQ=255)          cACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCcac                                                                 <  2:623607/81‑1 (MQ=255)             ggTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGa                                                              >  2:623643/1‑81 (MQ=255)               tCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACt                                                            <  1:623608/81‑1 (MQ=255)                cAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTg                                                           >  2:623663/1‑81 (MQ=255)                  gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCt                                                         <  2:623609/81‑1 (MQ=255)                  gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCt                                                         >  1:623640/1‑81 (MQ=255)                    gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTcc                                                       <  1:623610/81‑1 (MQ=255)                    gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTc                                                        >  1:623648/1‑80 (MQ=255)                        tattatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATaa                                                   >  1:623656/1‑81 (MQ=255)                          ttatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAAt                                                  <  1:623611/80‑1 (MQ=255)                             tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCa                                               <  2:623612/80‑1 (MQ=255)                             tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAc                                              >  1:623660/1‑81 (MQ=255)                             tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAc                                              >  2:623653/1‑81 (MQ=255)                               gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAccc                                            <  2:623613/81‑1 (MQ=255)                               gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAccc                                            <  1:623614/81‑1 (MQ=255)                                aaGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCt                                           <  1:623615/81‑1 (MQ=255)                                 aGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCtt                                          <  1:623616/81‑1 (MQ=255)                                    tGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGt                                       <  2:623617/81‑1 (MQ=255)                                    tGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGt                                       <  1:623618/81‑1 (MQ=255)                                      cAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTc                                      <  2:623619/80‑1 (MQ=255)                                      cAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCt                                     >  2:623665/1‑81 (MQ=255)                                           aTTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGt                                >  2:623669/1‑81 (MQ=255)                                              gCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGa                             >  2:623647/1‑81 (MQ=255)                                               ccACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGa                             <  2:623620/80‑1 (MQ=255)                                                    cgTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCt                       <  1:623621/81‑1 (MQ=255)                                                       aaaaCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg                    >  2:623658/1‑81 (MQ=255)                                                        aaaCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg                    >  2:623655/1‑80 (MQ=255)                                                          aCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCAt                 <  1:623622/81‑1 (MQ=255)                                                             gACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgc              >  2:623657/1‑81 (MQ=255)                                                              aCATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgc              <  1:623623/80‑1 (MQ=255)                                                                aTCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCt           <  2:623624/81‑1 (MQ=255)                                                                    cgcATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAt       <  1:623625/81‑1 (MQ=255)                                                                    cgcATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAt       <  1:623626/81‑1 (MQ=255)                                                                         atCGCCTAAATTCGCCACAGACTGCTCCATAATTCACTCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAc  >  2:623667/1‑81 (MQ=255)                                                                             |                                                                             CCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAC  >  NC_000913/3339964‑3340116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑