Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,205	T→A	V494E (GTG→GAG)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,205	0	T	A	100.0%	111.1 / NA	31	V494E (GTG→GAG)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/19);  total (12/19)

GGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGA  >  NC_000913/3356128‑3356280                                                                              |                                                                            ggACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGTGATGATACCCCATTCGCCGAGct                                                                          >  2:627658/1‑81 (MQ=255)  gACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGc                                                                           <  2:627616/79‑1 (MQ=255)       cGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTcc                                                                      <  1:627617/79‑1 (MQ=255)       cGTAATTCGAGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAg                                                                    >  2:627650/1‑81 (MQ=255)        gTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGt                                                                   <  2:627618/81‑1 (MQ=255)             tCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGcc                                                              >  2:627657/1‑81 (MQ=255)              cgcgTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGcc                                                              <  1:627619/80‑1 (MQ=255)               gcgTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCcgc                                                            <  2:627620/81‑1 (MQ=255)                 gTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCgcgc                                                          <  1:627621/81‑1 (MQ=255)                  tACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCa                                                         >  1:627653/1‑81 (MQ=255)                    cTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCat                                                        >  1:627661/1‑80 (MQ=255)                    cTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCat                                                        <  2:627622/80‑1 (MQ=255)                             aaCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGac                                              >  2:627656/1‑81 (MQ=255)                              aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact                                             <  1:627623/81‑1 (MQ=255)                              aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact                                             <  1:627625/81‑1 (MQ=255)                              aCGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGact                                             <  1:627624/81‑1 (MQ=255)                                  tCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACtt                                         <  2:627626/81‑1 (MQ=255)                                        aGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGc                                     >  2:627660/1‑79 (MQ=255)                                        aGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCca                                   <  2:627627/81‑1 (MQ=255)                                           ggtcggtTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCcag                                  >  2:627663/1‑79 (MQ=255)                                                gtTCGATGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTg                           <  1:627628/81‑1 (MQ=255)                                                      tGGGCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAgg                     <  1:627629/81‑1 (MQ=255)                                                        ggCGATGATACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTg                   <  1:627630/81‑1 (MQ=255)                                                            atgatACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGATTACTTCCGCCAGCAGTTTGCCCAGGTGACt                >  2:627665/1‑80 (MQ=255)                                                               atACCCCATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAAc             <  1:627631/80‑1 (MQ=255)                                                                   cccATTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGcc        <  1:627632/81‑1 (MQ=255)                                                                      aTTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAAt     >  1:627681/1‑81 (MQ=255)                                                                      aTTCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTCCCAAGGTGACTAACCCGCCAAt     <  2:627633/81‑1 (MQ=255)                                                                        tCGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATc    >  2:627676/1‑80 (MQ=255)                                                                         cGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGa  >  1:627673/1‑81 (MQ=255)                                                                         cGCCGAGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTACCCAGGTGACTAACCCGCCAATCg   <  2:627634/80‑1 (MQ=255)                                                                              |                                                                            GGACTCCGTAATTCGCGTACTGGGCGAAAACGGTCAGGAAGCGGTCGGTTCGATGGGCGATGATACCCCATTCGCCGTGCTCTCCAGTCAGCCGCGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGA  >  NC_000913/3356128‑3356280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑