Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,105	T→G	H110P (CAC→CCC)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,105	0	T	G	100.0%	87.3 / NA	25	H110P (CAC→CCC)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/11);  total (14/11)

CCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTC  >  NC_000913/334027‑334180                                                                               |                                                                            ccGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGgtgggt                                                                           <  2:74172/81‑1 (MQ=255)  cGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGtt                                                                          <  2:74173/81‑1 (MQ=255)   gTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGttt                                                                         <  1:74174/81‑1 (MQ=255)   gTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGttt                                                                         <  2:74175/81‑1 (MQ=255)     tataCACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTgg                                                                       >  1:74208/1‑81 (MQ=255)               ggtggtGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAag                                                             >  2:74206/1‑81 (MQ=255)                gtggtgACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAag                                                             >  2:74205/1‑80 (MQ=255)                      aCGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGa                                                      >  1:74213/1‑81 (MQ=255)                        ggTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGAtt                                                    <  1:74176/81‑1 (MQ=255)                        ggTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGAt                                                     >  1:74214/1‑80 (MQ=255)                        ggTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGAt                                                     >  1:74216/1‑80 (MQ=255)                              cTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATAc                                               >  1:74217/1‑80 (MQ=255)                                 gCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAAt                                           >  2:74226/1‑81 (MQ=255)                                 gCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAAt                                           >  2:74209/1‑81 (MQ=255)                                  cAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATg                                          <  2:74178/81‑1 (MQ=255)                                  cAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATg                                          <  2:74177/81‑1 (MQ=255)                                            aCTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACAcgcg                                >  1:523608/1‑81 (MQ=255)                                               gCTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCtt                             >  1:74203/1‑81 (MQ=255)                                                cTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTc                            <  2:74179/81‑1 (MQ=255)                                                cTTTTTCAGCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTc                            >  2:74210/1‑81 (MQ=255)                                                        gCACCTGTAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCt                    <  2:74180/81‑1 (MQ=255)                                                               tAACAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAAt             <  1:74181/81‑1 (MQ=255)                                                                 aCAAGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATa            <  2:74182/80‑1 (MQ=255)                                                                   aaGCGTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTa         >  2:74219/1‑81 (MQ=255)                                                                       gTGCGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGa     >  2:74211/1‑81 (MQ=255)                                                                          cGGTGGGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTc  <  1:74183/81‑1 (MQ=255)                                                                               |                                                                            CCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTC  >  NC_000913/334027‑334180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑