Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,546	G→T	N139K (AAC→AAA)	yrdD ←	putative DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,546	0	G	T	100.0%	69.3 / NA	20	N139K (AAC→AAA)	yrdD	putative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/8);  total (12/8)

TTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGAC  >  NC_000913/3431470‑3431620                                                                              |                                                                           ttACACCCTGCGCGGCTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTa                                                                         <  1:645151/80‑1 (MQ=255)   acacCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATg                                                                      >  2:645182/1‑81 (MQ=255)    cacCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATgg                                                                     >  1:645174/1‑81 (MQ=255)     acCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATgg                                                                     >  1:645175/1‑80 (MQ=255)      cccTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTTGAATTTAATGGc                                                                    <  2:645152/81‑1 (MQ=255)          gcgcGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAAtt                                                               >  2:645172/1‑81 (MQ=255)              ggTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGaca                                                           >  2:645214/1‑81 (MQ=255)                             gAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGAt                                             >  2:645179/1‑80 (MQ=255)                                   cGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAaga                                      <  1:645153/81‑1 (MQ=255)                                    ggATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAagag                                     >  1:645184/1‑81 (MQ=255)                                         aTGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGaaa                                >  2:645191/1‑81 (MQ=255)                                           gACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATg                              >  2:645193/1‑81 (MQ=255)                                             cacTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGtt                            <  1:645154/81‑1 (MQ=255)                                              acTCAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGttt                           >  2:645180/1‑81 (MQ=255)                                                 cAGGGCATTCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGc                        >  1:645183/1‑81 (MQ=255)                                                       aTTCTCCAGCTATGGG‑TTTGAATCTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAAc                  >  1:645186/1‑81 (MQ=255)                                                        ttCTCCAGCTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACg                 <  1:762965/81‑1 (MQ=255)                                                                cTATGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAgcggc         <  2:645155/81‑1 (MQ=255)                                                                   tGGG‑TTTGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAgcggcg        <  2:645156/79‑1 (MQ=255)                                                                        tttGAATTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGAc  <  1:645157/81‑1 (MQ=255)                                                                              |                                                                           TTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGG‑TTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGAC  >  NC_000913/3431470‑3431620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑