Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,490,253	T→C	intergenic (‑73/+13)	argD ← / ← pabA	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase/aminodeoxychorismate synthase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,490,253	0	T	C	100.0%	120.8 / NA	34	intergenic (‑73/+13)	argD/pabA	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase/aminodeoxychorismate synthase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/18);  total (16/18)

ATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGG  >  NC_000913/3490179‑3490329                                                                           |                                                                             aTCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAAt                                                                        >  1:658925/1‑81 (MQ=255)      aTGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGa                                                                    >  1:658915/1‑80 (MQ=255)              ccTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGa                                                           <  2:658873/81‑1 (MQ=255)               cTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGAt                                                          <  2:658874/81‑1 (MQ=255)                   tACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAg                                                      >  1:658937/1‑81 (MQ=255)                    aCGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAg                                                      <  2:658875/80‑1 (MQ=255)                         tAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAAtt                                                <  1:658876/81‑1 (MQ=255)                         tAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAAt                                                 >  1:658902/1‑80 (MQ=255)                           aaCAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAg                                              <  2:658877/81‑1 (MQ=255)                           aaCAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAg                                              >  1:658916/1‑81 (MQ=255)                                   tgAAATTATAACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGt                                      <  2:658878/81‑1 (MQ=255)                                         tataACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGt                                >  2:658914/1‑81 (MQ=255)                                         tataACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGt                                >  2:658927/1‑81 (MQ=255)                                          ataACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGt                                <  2:658879/80‑1 (MQ=255)                                           taACCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTc                               <  2:658880/80‑1 (MQ=255)                                             aCCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCtt                            <  2:658882/81‑1 (MQ=255)                                             aCCACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCtt                            <  1:658881/81‑1 (MQ=255)                                              ccACAAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTg                           <  2:658883/81‑1 (MQ=255)                                                 caAAATATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTc                        >  2:658905/1‑81 (MQ=255)                                                  aaaaTATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCg                       >  1:658917/1‑81 (MQ=255)                                                    aaTATGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCt                     <  2:658884/81‑1 (MQ=255)                                                       atGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCt                     >  2:658918/1‑78 (MQ=255)                                                        tGCATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAg                  <  2:658885/80‑1 (MQ=255)                                                          cATAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAAt               <  1:658886/81‑1 (MQ=255)                                                           aTAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATa              >  2:658919/1‑81 (MQ=255)                                                            tAAAAAATCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATAc             >  2:658935/1‑81 (MQ=255)                                                              aaaaaTCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACtt           >  1:658923/1‑81 (MQ=255)                                                               aaaaTCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACttt          <  1:658887/81‑1 (MQ=255)                                                                 aaTCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCt        <  1:658888/81‑1 (MQ=255)                                                                  aTCACTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCtg       >  2:658934/1‑81 (MQ=255)                                                                     aCTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCtggat    <  2:658889/81‑1 (MQ=255)                                                                      cTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCtggatg   <  2:658890/81‑1 (MQ=255)                                                                      cTAAACGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCtggatg   >  2:658931/1‑81 (MQ=255)                                                                        aaaCGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCtggatgg  >  2:658932/1‑80 (MQ=255)                                                                           |                                                                             ATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGG  >  NC_000913/3490179‑3490329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑