Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,540,248	T→C	L29P (CTT→CCT)	feoA →	ferrous iron transport protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,540,248	0	T	C	100.0%	88.0 / NA	25	L29P (CTT→CCT)	feoA	ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/15);  total (10/15)

ACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGT  >  NC_000913/3540172‑3540324                                                                             |                                                                             acTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGc                                                                          <  1:671572/81‑1 (MQ=255)  cTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCa                                                                         >  2:671607/1‑81 (MQ=255)     cAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCAt                                                                        <  2:671573/79‑1 (MQ=255)       gATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTa                                                                    <  2:671574/81‑1 (MQ=255)          aCTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCt                                                                 >  2:671618/1‑81 (MQ=255)          aCTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGCCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCt                                                                 >  1:671601/1‑81 (MQ=255)           cTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCt                                                                 <  2:671575/80‑1 (MQ=255)               gTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGctc                                                            <  1:671576/81‑1 (MQ=255)                  gAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGctcctc                                                         <  1:671577/81‑1 (MQ=255)                     aaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCttt                                                      <  1:671578/81‑1 (MQ=255)                       tCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTa                                                    <  1:671579/81‑1 (MQ=255)                             gCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATgtgg                                              >  1:671621/1‑81 (MQ=255)                             gCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATgtgg                                              >  1:671612/1‑81 (MQ=255)                                 ttCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTgc                                           <  2:900954/80‑1 (MQ=255)                                              aGCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTc                             <  1:671580/81‑1 (MQ=255)                                               gCCCGGCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCg                            >  1:671616/1‑81 (MQ=255)                                                    gCATATCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGAc                       <  2:671581/81‑1 (MQ=255)                                                         tCGCCAAAAACTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCa                   <  2:671582/80‑1 (MQ=255)                                                              aaaaaCTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCata             >  2:671614/1‑81 (MQ=255)                                                                  aCTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCg          >  2:671620/1‑80 (MQ=255)                                                                  aCTGCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGa         <  2:671583/81‑1 (MQ=255)                                                                     gCTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAc      >  2:671617/1‑81 (MQ=255)                                                                      cTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAcc     <  2:671584/81‑1 (MQ=255)                                                                      cTTTCTCCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAAc      <  2:671585/80‑1 (MQ=255)                                                                         tctcCTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCcgt  >  2:671627/1‑81 (MQ=255)                                                                             |                                                                             ACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGT  >  NC_000913/3540172‑3540324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑