Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,550,685	T→C	N597D (AAC→GAC)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,550,685	0	T	C	100.0%	149.4 / NA	41	N597D (AAC→GAC)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

AGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCC  >  NC_000913/3550609‑3550760                                                                             |                                                                            aGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGAt                                                                          >  1:674303/1‑80 (MQ=255)  gTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCa                                                                        >  1:674314/1‑81 (MQ=255)  gTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCa                                                                        <  1:674259/81‑1 (MQ=255)        ccgccgccgAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCa                                                                   <  2:674260/80‑1 (MQ=255)          gccgccgAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGc                                                                 >  2:674311/1‑80 (MQ=255)             gccgAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACt                                                             <  1:674261/81‑1 (MQ=255)               cgAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACttt                                                           <  1:674262/81‑1 (MQ=255)                gAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTttg                                                          >  2:674306/1‑81 (MQ=255)                 aaaCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTttgt                                                         >  2:674312/1‑81 (MQ=255)                  aaCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTttgtt                                                        >  1:674302/1‑81 (MQ=255)                   aCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTttgttg                                                       <  2:674263/81‑1 (MQ=255)                   aCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTttgttg                                                       <  2:674264/81‑1 (MQ=255)                    cGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGa                                                      >  1:674318/1‑81 (MQ=255)                     gCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGAt                                                     <  1:674265/81‑1 (MQ=255)                       aataatCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCg                                                   <  1:674266/81‑1 (MQ=255)                              cGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGat                                            <  2:674267/81‑1 (MQ=255)                               ggCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGat                                            >  1:674307/1‑80 (MQ=255)                                     aaCACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttc                                     <  2:674269/81‑1 (MQ=255)                                     aaCACCACCTTCAACTTATCGCCAACCAGCGAATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttc                                     <  2:674268/81‑1 (MQ=255)                                        accaccTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                                  <  1:674270/81‑1 (MQ=255)                                         ccaccTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCg                                 <  2:674271/81‑1 (MQ=255)                                          caccTTCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGc                                >  2:674330/1‑81 (MQ=255)                                              ttCAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAgg                            >  1:674309/1‑81 (MQ=255)                                                cAACTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt                           <  1:674272/80‑1 (MQ=255)                                                 aaCTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta                          <  2:674273/80‑1 (MQ=255)                                                  aCTTATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt                        <  2:674274/81‑1 (MQ=255)                                                    ttATCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGtagtag                      >  1:674310/1‑81 (MQ=255)                                                      aTCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGtagtag                      <  1:674275/79‑1 (MQ=255)                                                      aTCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc                     <  1:674276/80‑1 (MQ=255)                                                       tCGCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGccc                   <  1:674277/81‑1 (MQ=255)                                                         gCCAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCgg                 >  1:674304/1‑81 (MQ=255)                                                           cAACCAGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTg               <  1:674278/81‑1 (MQ=255)                                                                aGCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCt          >  2:674321/1‑81 (MQ=255)                                                                 gCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCtt         <  1:674280/81‑1 (MQ=255)                                                                 gCGGATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCtt         <  2:674279/81‑1 (MQ=255)                                                                    gATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcg      >  1:674316/1‑81 (MQ=255)                                                                    gATCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcg      >  1:674317/1‑81 (MQ=255)                                                                     aTCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcgc     <  1:674281/81‑1 (MQ=255)                                                                     aTCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcgc     <  2:674282/81‑1 (MQ=255)                                                                     aTCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcgc     >  2:674313/1‑81 (MQ=255)                                                                     aTCGTTGTCGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTcgc     >  2:674319/1‑81 (MQ=255)                                                                        gttgtcGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGcc  >  1:674323/1‑81 (MQ=255)                                                                             |                                                                            AGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCC  >  NC_000913/3550609‑3550760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑