Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	61,164	C→T	A701T (GCA→ACA)	rapA ←	RNA polymerase‑binding ATPase and RNAP recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	61,164	0	C	T	100.0%	138.4 / NA	38	A701T (GCA→ACA)	rapA	RNA polymerase‑binding ATPase and RNAP recycling factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

ATATCGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGCCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAGCGCTT  >  NC_000913/61091‑61241                                                                          |                                                                              atatCGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCt                                                                        <  2:14019/81‑1 (MQ=255)    tCGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCttt                                                                      <  1:14020/80‑1 (MQ=255)     cGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCttttt                                                                    >  2:14073/1‑81 (MQ=255)     cGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCttttt                                                                    >  1:14090/1‑81 (MQ=255)      gAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTc                                                                   <  1:14021/81‑1 (MQ=255)         cAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGcc                                                                <  2:14022/81‑1 (MQ=255)          aGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCa                                                               <  2:14023/81‑1 (MQ=255)          aGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCa                                                               >  1:14093/1‑81 (MQ=255)           ggTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCAc                                                              >  2:14079/1‑81 (MQ=255)               cATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGtt                                                          >  1:14082/1‑81 (MQ=255)                aTGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGtt                                                          <  2:14025/80‑1 (MQ=255)                aTGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGtt                                                          <  2:14024/80‑1 (MQ=255)                            aTCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTc                                              >  1:14086/1‑80 (MQ=255)                             tCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTc                                              >  1:14105/1‑79 (MQ=255)                                ggTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCcag                                           >  1:14092/1‑79 (MQ=255)                                       aTCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGt                                   >  1:14085/1‑80 (MQ=255)                                         cGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCTCCGTTGGAGTGGATTTCCAGCAGGCGGTCAc                                >  2:14080/1‑81 (MQ=255)                                         cGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCAc                                >  2:14087/1‑81 (MQ=255)                                          gTCATCCTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCAc                                <  1:14026/80‑1 (MQ=255)                                                cTGCTCTTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTTGATTTCCAGCAGGCGGTCACGACCCTg                         >  1:14091/1‑81 (MQ=255)                                                   ctctTCAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGtt                       <  2:14027/80‑1 (MQ=255)                                                        cAATGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAg                   <  2:14028/79‑1 (MQ=255)                                                         aaTGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCtg                <  2:14029/81‑1 (MQ=255)                                                         aaTGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCtg                <  2:14030/81‑1 (MQ=255)                                                          aTGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCtgt               >  1:14104/1‑81 (MQ=255)                                                          aTGCTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCtgt               >  1:14089/1‑81 (MQ=255)                                                             cTTTCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGc             <  1:14031/80‑1 (MQ=255)                                                                tCTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCttt          <  2:14032/80‑1 (MQ=255)                                                                 cTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCttt          >  2:14102/1‑79 (MQ=255)                                                                 cTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTc         <  2:14033/80‑1 (MQ=255)                                                                 cTGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTc         <  2:14034/80‑1 (MQ=255)                                                                  tGCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAg       >  2:14072/1‑81 (MQ=255)                                                                   gCCAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAgc      >  2:14083/1‑81 (MQ=255)                                                                    ccAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCATCAGGCGGTCACGCCCCTGTTCCAGCTGTGCTTTCAgcg     >  1:14088/1‑81 (MQ=255)                                                                    ccAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAg       <  1:14035/79‑1 (MQ=255)                                                                    ccAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAg       <  1:14036/79‑1 (MQ=255)                                                                     cAGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAgcgc    >  1:14100/1‑81 (MQ=255)                                                                      aGTGTCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAGCGCt   <  2:14037/81‑1 (MQ=255)                                                                       gtgtCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAGCGCtt  <  1:14038/81‑1 (MQ=255)                                                                          |                                                                              ATATCGAACAGGTTCATGGCGAAGGCGATCAGGTTGGTATCGTCATCCTGCTCTTCAATGCTTTCTGCCAGTGCCTGGGCTTTTTCGCCACCGTTGGAGTGGATTTCCAGCAGGCGGTCACGACCCTGTTCCAGCTGTGCTTTCAGCGCTT  >  NC_000913/61091‑61241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑