Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	341,434	C→G	P104A (CCG→GCG)	yahJ →	putative deaminase with metallo‑dependent hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	341,434	0	C	G	100.0%	80.2 / NA	23	P104A (CCG→GCG)	yahJ	putative deaminase with metallo‑dependent hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/13);  total (10/13)

CGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACC  >  NC_000913/341359‑341505                                                                            |                                                                        cgcCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCAc                                                                    <  1:75789/81‑1 (MQ=255)    cAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTAt                                                                 >  1:75829/1‑81 (MQ=255)          gTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCt                                                           <  2:75790/81‑1 (MQ=255)             gagaTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTgg                                                         <  1:75792/80‑1 (MQ=255)             gagaTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGc                                                        <  1:75791/81‑1 (MQ=255)                 ttCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCAGTATGACGCTGGCGGt                                                     >  2:75833/1‑80 (MQ=255)                         ggCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtg                                            >  2:75845/1‑81 (MQ=255)                          gCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtgc                                           <  2:75793/81‑1 (MQ=255)                                ttGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCcca                                     >  2:75917/1‑81 (MQ=255)                                 tGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccac                                    >  2:75851/1‑81 (MQ=255)                                     gCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCaccacc                                >  2:75837/1‑81 (MQ=255)                                      cccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCAccc                               <  1:75794/81‑1 (MQ=255)                                       ccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCcg                              >  2:75844/1‑81 (MQ=255)                                       ccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCcg                              <  1:75795/81‑1 (MQ=255)                                                    aaGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATAtt                 >  2:75860/1‑81 (MQ=255)                                                     aGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATAtt                 >  2:75849/1‑80 (MQ=255)                                                       cTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAt              <  2:75796/81‑1 (MQ=255)                                                          cATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAtctc           <  2:75797/81‑1 (MQ=255)                                                           atacGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCg          <  2:75798/78‑1 (MQ=255)                                                           aTCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCg          <  2:75799/81‑1 (MQ=255)                                                                gACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaa      >  1:75854/1‑80 (MQ=255)                                                                   gCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAAcc  <  2:75800/81‑1 (MQ=255)                                                                   gCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAAcc  <  2:75801/81‑1 (MQ=255)                                                                            |                                                                        CGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACC  >  NC_000913/341359‑341505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑