Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,594,418	C→G	V355L (GTG→CTG)	livM ←	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,594,418	0	C	G	100.0%	198.5 / NA	54	V355L (GTG→CTG)	livM	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/27);  total (27/27)

CGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCACCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAA  >  NC_000913/3594342‑3594493                                                                             |                                                                            cGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACt                                                                         <  2:684900/81‑1 (MQ=255)  gCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTa                                                                        >  2:684959/1‑81 (MQ=255)     tCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCg                                                                     <  2:684901/81‑1 (MQ=255)       aaCTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGc                                                                    <  1:684902/80‑1 (MQ=255)             cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac                                                              >  2:684981/1‑80 (MQ=255)             cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac                                                              >  2:684964/1‑80 (MQ=255)             cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac                                                              >  1:685006/1‑80 (MQ=255)              gcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcaca                                                             >  1:684996/1‑80 (MQ=255)              gcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACaa                                                            <  1:684903/81‑1 (MQ=255)               cgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACaaa                                                           >  2:684973/1‑81 (MQ=255)                gACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAAc                                                          >  2:684988/1‑81 (MQ=255)                gACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAAc                                                          <  2:684904/81‑1 (MQ=255)                  caccacCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGc                                                        >  2:684984/1‑81 (MQ=255)                        caGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTc                                                  <  2:684905/81‑1 (MQ=255)                         aGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCg                                                 >  2:684997/1‑81 (MQ=255)                            aaaaTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCa                                              <  1:684906/81‑1 (MQ=255)                             aaaTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCaa                                             <  1:684907/81‑1 (MQ=255)                               aTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAg                                           >  1:684980/1‑81 (MQ=255)                                 tGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGt                                         >  1:685000/1‑81 (MQ=255)                                   ccgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTg                                        <  2:684908/80‑1 (MQ=255)                                    cgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTg                                        >  1:685063/1‑79 (MQ=255)                                    cgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGaa                                      <  1:684909/81‑1 (MQ=255)                                     gccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAg                                     >  1:684983/1‑81 (MQ=255)                                      ccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAgg                                    <  2:684910/81‑1 (MQ=255)                                       cAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGa                                   >  2:684982/1‑81 (MQ=255)                                       cAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGAGAAGGa                                   >  2:684972/1‑81 (MQ=255)                                        aGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGAt                                  <  2:684911/81‑1 (MQ=255)                                         gAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGAtt                                 >  2:684995/1‑81 (MQ=255)                                           aTCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTcc                               <  1:684913/81‑1 (MQ=255)                                           aTCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTcc                               <  1:684912/81‑1 (MQ=255)                                            tCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCg                              <  1:684914/81‑1 (MQ=255)                                             cACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCgg                             >  2:684987/1‑81 (MQ=255)                                              aCCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCggg                            >  2:684990/1‑81 (MQ=255)                                                 gCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCt                          >  2:684985/1‑80 (MQ=255)                                                  cAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa                        >  2:684978/1‑81 (MQ=255)                                                  cAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa                        <  1:684915/81‑1 (MQ=255)                                                   aaaTTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa                        >  1:684974/1‑80 (MQ=255)                                                        gCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGc                  >  2:684998/1‑81 (MQ=255)                                                         cGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGc                  <  2:684916/80‑1 (MQ=255)                                                           aGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCt               <  1:684917/81‑1 (MQ=255)                                                            gCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTg              <  1:684918/81‑1 (MQ=255)                                                              ccATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAc            <  2:684919/81‑1 (MQ=255)                                                              ccATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAc            <  1:684920/81‑1 (MQ=255)                                                                aTACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAcgc          >  1:685014/1‑81 (MQ=255)                                                                 tACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAcgcg         <  1:684921/81‑1 (MQ=255)                                                                   ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACgcgc        <  1:684923/80‑1 (MQ=255)                                                                   ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACgcgc        <  1:684922/80‑1 (MQ=255)                                                                   ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgcc       <  2:684925/81‑1 (MQ=255)                                                                   ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgcc       <  1:684924/81‑1 (MQ=255)                                                                    cgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgccg      >  2:685008/1‑81 (MQ=255)                                                                     gccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgccgc     <  2:684926/81‑1 (MQ=255)                                                                       cgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCa    >  1:684991/1‑80 (MQ=255)                                                                       cgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCa    >  2:684994/1‑80 (MQ=255)                                                                        gAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCaaa  >  1:685003/1‑81 (MQ=255)                                                                             |                                                                            CGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCACCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAA  >  NC_000913/3594342‑3594493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑