Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,464	C→T	A33T (GCG→ACG)	livK ←	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,464	0	C	T	100.0%	86.0 / NA	24	A33T (GCG→ACG)	livK	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/16);  total (8/16)

CATTAATGTCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCC  >  NC_000913/3597389‑3597529                                                                            |                                                                  cATTAATGTCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCTCACTGGGCAATCGGGCCGGACATCGTGCCGa                                                              >  2:685810/1‑81 (MQ=255)  aTTAATGTCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGAc                                                             <  2:685768/81‑1 (MQ=255)       tGTCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACgg                                                        <  2:685769/81‑1 (MQ=255)         tCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCg                                                      <  2:685770/81‑1 (MQ=255)             tAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGAc                                                    <  1:685771/79‑1 (MQ=255)             tAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACtt                                                  >  1:685817/1‑81 (MQ=255)              aaTTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACtt                                                  <  2:685772/80‑1 (MQ=255)               aTTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACttt                                                 >  2:685815/1‑80 (MQ=255)                   ccTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAatat                                            >  1:685813/1‑81 (MQ=255)                             gcCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCa                                   >  2:685820/1‑80 (MQ=255)                               cGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAg                                <  2:685773/81‑1 (MQ=255)                                gTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGc                               >  1:685818/1‑81 (MQ=255)                                 ttAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCg                              <  2:685774/81‑1 (MQ=255)                                   aaaTTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGgt                            >  1:685816/1‑81 (MQ=255)                                         cATATCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGa                      <  2:685775/81‑1 (MQ=255)                                          atatCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGaa                     <  1:685776/81‑1 (MQ=255)                                             tCGCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAAt                   <  1:685777/80‑1 (MQ=255)                                               gCCCCACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGc                <  2:685778/81‑1 (MQ=255)                                                   cACTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGt            <  2:685779/81‑1 (MQ=255)                                                    aCTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTg           <  1:685780/81‑1 (MQ=255)                                                     cTGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGc          <  1:685781/81‑1 (MQ=255)                                                      tGGGCAATCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGc          >  2:685823/1‑80 (MQ=255)                                                           aaTCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAatcat    <  1:685782/81‑1 (MQ=255)                                                             tCGGGCCGGACATCGTGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATcc  <  1:685783/81‑1 (MQ=255)                                                                            |                                                                  CATTAATGTCTTTAATTGCCTGACGCGCGCCGTTAAATTCCATATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCC  >  NC_000913/3597389‑3597529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑