Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,711,450	C→A	M186I (ATG→ATT)	yhjX ←	putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,711,450	0	C	A	100.0%	75.1 / NA	22	M186I (ATG→ATT)	yhjX	putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/10);  total (12/10)

CGCATCGACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTCATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAGAC  >  NC_000913/3711373‑3711520                                                                              |                                                                       cGCATCGACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAAtt                                                                     <  2:713564/81‑1 (MQ=255)  gCATCGACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTa                                                                    <  2:713565/81‑1 (MQ=255)    aTCGACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAAc                                                                  >  1:713609/1‑81 (MQ=255)       gACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGtt                                                               >  1:713598/1‑81 (MQ=255)                aGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGaaa                                                      <  1:713566/81‑1 (MQ=255)                       aaTCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAatca                                               >  2:713605/1‑81 (MQ=255)                              ctcCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAg                                        >  1:713602/1‑81 (MQ=255)                                  accacaccaTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCa                                    <  2:713567/81‑1 (MQ=255)                                   ccacaccaTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCaa                                   <  1:713568/81‑1 (MQ=255)                                     acaccaTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATc                                 >  1:713618/1‑81 (MQ=255)                                     acaccaTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATc                                 <  2:713569/81‑1 (MQ=255)                                     acaccaTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATc                                 <  1:713570/81‑1 (MQ=255)                                          aTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTcc                            <  2:713571/81‑1 (MQ=255)                                          aTTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTcc                            >  2:713608/1‑81 (MQ=255)                                            tGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTccc                           >  1:713626/1‑80 (MQ=255)                                             gCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTcccc                          <  2:713572/80‑1 (MQ=255)                                                    ttCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATcac                   >  1:713611/1‑80 (MQ=255)                                                    ttCACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATcac                   <  2:713573/80‑1 (MQ=255)                                                      cACTTCCTGTTTTGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATcaca                  >  2:713607/1‑79 (MQ=255)                                                                 ttGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTc       >  2:713606/1‑79 (MQ=255)                                                                  tGGTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTc       >  2:713610/1‑78 (MQ=255)                                                                    gTGCGTCTTTAATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAgac  >  1:713612/1‑81 (MQ=255)                                                                              |                                                                       CGCATCGACTCTGCCAGCGTGTAATCTTTCTCCACCACACCATTGCTGGTTTTCACTTCCTGTTTTGGTGCGTCTTTCATTAACGTTGCGCCGAAAACAATCATCAACAGCGCAATCGCTCCCCAAATCACAAAGGTTTTTTCCAGAC  >  NC_000913/3711373‑3711520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑