Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,795,757 G→C A277P (GCG→CCG)  waaF → ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,795,7570GC100.0% 154.0 / NA 43A277P (GCG→CCG) waaFADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/29);  total (14/29)

CAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAG  >  NC_000913/3795679‑3795832
                                                                              |                                                                           
caGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccg                                                                           <  2:734285/81‑1 (MQ=255)
  gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                         <  2:734287/81‑1 (MQ=255)
  gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                         <  2:734286/81‑1 (MQ=255)
       aTCAAGCGGTTATCCTGAATGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTc                                                                     <  1:734288/80‑1 (MQ=255)
         cAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAAt                                                                  >  2:734356/1‑81 (MQ=255)
          aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                 <  2:734289/81‑1 (MQ=255)
             cGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTc                                                              >  1:734341/1‑81 (MQ=255)
                  aTCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTg                                                         >  1:734346/1‑81 (MQ=255)
                   tCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTgg                                                        <  1:734290/81‑1 (MQ=255)
                   tCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTgg                                                        >  2:734361/1‑81 (MQ=255)
                     cTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGtt                                                      <  1:734291/81‑1 (MQ=255)
                     cTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGt                                                       <  2:734292/80‑1 (MQ=255)
                        aTTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGcc                                                   >  1:734351/1‑81 (MQ=255)
                        aTTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGc                                                    <  1:734293/80‑1 (MQ=255)
                             aGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTa                                              >  2:734347/1‑81 (MQ=255)
                                cTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATgg                                           >  2:734358/1‑81 (MQ=255)
                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGGTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTcc                                        <  2:734295/81‑1 (MQ=255)
                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGt                                          <  2:734294/79‑1 (MQ=255)
                                   tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGt                                          <  1:734296/79‑1 (MQ=255)
                                     aaGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCg                                       <  2:734297/80‑1 (MQ=255)
                                      aGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAg                                     <  2:734298/81‑1 (MQ=255)
                                       gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGt                                    <  1:734299/81‑1 (MQ=255)
                                         cATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAg                                  <  1:734300/81‑1 (MQ=255)
                                            tGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGccc                               >  1:734336/1‑81 (MQ=255)
                                             gTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTAAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGccc                               <  1:734301/80‑1 (MQ=255)
                                              tCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCgg                             <  1:734303/81‑1 (MQ=255)
                                              tCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCg                              <  2:734302/80‑1 (MQ=255)
                                                 cTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACt                          <  1:734304/81‑1 (MQ=255)
                                                    aCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTc                        <  2:734305/80‑1 (MQ=255)
                                                        ttCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAcc                   <  2:734306/81‑1 (MQ=255)
                                                         tCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccg                  >  2:734350/1‑81 (MQ=255)
                                                             gCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc                <  2:734307/79‑1 (MQ=255)
                                                             gCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc                <  1:734308/79‑1 (MQ=255)
                                                             gCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACcgccgc              >  1:734360/1‑81 (MQ=255)
                                                              ccTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCt             <  1:734310/81‑1 (MQ=255)
                                                              ccTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCt             <  2:734309/81‑1 (MQ=255)
                                                               cTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTa            <  2:734311/81‑1 (MQ=255)
                                                                  aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTAt           <  1:734314/79‑1 (MQ=255)
                                                                  aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATc          <  2:734312/80‑1 (MQ=255)
                                                                  aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATc          <  1:734313/80‑1 (MQ=255)
                                                                   tGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATccc        <  2:734315/81‑1 (MQ=255)
                                                                    gCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCa       >  1:734365/1‑81 (MQ=255)
                                                                         ttGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAg  >  1:734373/1‑81 (MQ=255)
                                                                         ttGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAg  <  1:734316/81‑1 (MQ=255)
                                                                         ttGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAg  >  2:734357/1‑81 (MQ=255)
                                                                         ttGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAg  >  2:734369/1‑81 (MQ=255)
                                                                              |                                                                           
CAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAG  >  NC_000913/3795679‑3795832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑