Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	358,748	A→G	intergenic (‑58/‑51)	cynR ← / → cynT	DNA‑binding transcriptional dual regulator CynR/carbonic anhydrase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	358,748	0	A	G	100.0%	111.6 / NA	32	intergenic (‑58/‑51)	cynR/cynT	DNA‑binding transcriptional dual regulator CynR/carbonic anhydrase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

TTGATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCT  >  NC_000913/358671‑358824                                                                              |                                                                             ttGATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCaa                                                                           >  2:79537/1‑81 (MQ=255) ttGATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCaa                                                                           <  1:79509/81‑1 (MQ=255) ttGATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCaa                                                                           <  2:79510/81‑1 (MQ=255)   gATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAAt                                                                          <  2:79511/80‑1 (MQ=255)    atatGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCg                                                                        <  1:79512/81‑1 (MQ=255)           gagagaGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGagta                                                                 <  2:79513/81‑1 (MQ=255)           gagagaGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGag                                                                   >  2:79543/1‑79 (MQ=255)             gagagCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGagtagt                                                               >  2:79545/1‑81 (MQ=255)                 gCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGc                                                           <  2:79514/81‑1 (MQ=255)                     tCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCctct                                                       >  2:79547/1‑81 (MQ=255)                       gCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCtctc                                                      <  1:79515/80‑1 (MQ=255)                         aaCCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCAtt                                                   >  2:79566/1‑81 (MQ=255)                          aCCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTc                                                  <  2:79516/81‑1 (MQ=255)                           ccTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTc                                                  >  2:79550/1‑80 (MQ=255)                            cTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCa                                                <  1:79517/81‑1 (MQ=255)                             tataAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTc                                                  >  1:79551/1‑78 (MQ=255)                               taAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCagag                                             <  1:79518/81‑1 (MQ=255)                                  gTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGagaca                                          <  2:79519/81‑1 (MQ=255)                                    aaaTCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGagacaga                                        >  1:79570/1‑81 (MQ=255)                                             ggAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGtt                                >  1:79565/1‑80 (MQ=255)                                              gAACTCATCATAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTaa                              <  2:79520/81‑1 (MQ=255)                                                      catAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGa                        >  1:79568/1‑79 (MQ=255)                                                        tAAATGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAaga                    <  1:79521/81‑1 (MQ=255)                                                         aaaTGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGaaa                      >  1:79558/1‑78 (MQ=255)                                                          aaTGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAgaga                  <  1:79522/81‑1 (MQ=255)                                                           aTGAGACTTTTACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGat                 >  2:79557/1‑81 (MQ=255)                                                                  ttttACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGAt          >  1:79554/1‑81 (MQ=255)                                                                    ttACCTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATg         <  1:79523/80‑1 (MQ=255)                                                                       ccTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGAtt     >  2:79546/1‑81 (MQ=255)                                                                       ccTTATGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGAtt     >  2:79559/1‑81 (MQ=255)                                                                           aTGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCt  <  2:79524/80‑1 (MQ=255)                                                                           aTGGCAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCt  >  2:79556/1‑80 (MQ=255)                                                                              |                                                                             TTGATATGTCGAGAGAGCATTCGCAACCTATAAGTAAATCCAATGGAACTCATCATAAATGAGACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCAGAGACAGACAGAGGTTAACGGTGAAAGAGATTATTGATGGATTCCT  >  NC_000913/358671‑358824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑