| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,814 | C→T | intergenic (‑46/+49) | pyrE ← / ← rph | orotate phosphoribosyltransferase/truncated RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,814 | 0 | C | T | 97.2% | 122.8 / ‑3.9 | 36 | intergenic (‑46/+49) | pyrE/rph | orotate phosphoribosyltransferase/truncated RNase PH |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (22/13); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
ATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGG > NC_000913/3815738‑3815888 | aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATtcat < 1:737968/81‑1 (MQ=255) cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAg > 1:738002/1‑81 (MQ=255) aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTc < 1:737969/81‑1 (MQ=255) aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGcc > 2:738001/1‑81 (MQ=255) aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGcc > 1:738020/1‑81 (MQ=255) ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTa > 2:738007/1‑80 (MQ=255) ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTa > 2:738003/1‑80 (MQ=255) tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAAt > 2:738009/1‑80 (MQ=255) tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGt > 2:738026/1‑81 (MQ=255) ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTg > 2:738016/1‑81 (MQ=255) gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGc < 2:737970/81‑1 (MQ=255) tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGc > 2:738027/1‑80 (MQ=255) ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGc > 1:738012/1‑79 (MQ=255) tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAg > 2:738029/1‑81 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAATCGCCTTAAAAATCAGTTTGCCAgc < 2:737971/80‑1 (MQ=255) gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc > 2:737997/1‑81 (MQ=255) cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc < 1:737972/81‑1 (MQ=255) cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCgccgcc < 1:737974/79‑1 (MQ=255) cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCgccgcc < 2:737973/79‑1 (MQ=255) gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc > 1:738025/1‑81 (MQ=255) gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc > 1:738008/1‑81 (MQ=255) ctcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt < 2:737975/81‑1 (MQ=255) ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc < 1:737976/80‑1 (MQ=255) tcATCTTACTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt > 2:738010/1‑81 (MQ=255) aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc < 1:737977/80‑1 (MQ=255) aCTTTTCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt < 1:737978/78‑1 (MQ=255) ttCTACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt < 1:737979/79‑1 (MQ=255) tACAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgatt > 1:738023/1‑81 (MQ=255) cAGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc > 1:738019/1‑80 (MQ=255) aGACAAAAAAAAGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga > 1:738011/1‑81 (MQ=255) aaaaGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc < 1:737980/81‑1 (MQ=255) aaaaGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc > 2:738014/1‑81 (MQ=255) aaaaGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc > 2:738015/1‑81 (MQ=255) aaGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg < 1:737981/81‑1 (MQ=255) aaGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCgg > 1:738028/1‑81 (MQ=255) aGGCGATTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg > 1:738018/1‑81 (MQ=255) | ATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGG > NC_000913/3815738‑3815888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |