Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,835,684	C→A	W82C (TGG→TGT)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,835,684	0	C	A	100.0%	175.6 / NA	48	W82C (TGG→TGT)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/27);  total (21/27)

GCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAG  >  NC_000913/3835609‑3835761                                                                            |                                                                              gCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACg                                                                           >  2:743434/1‑80 (MQ=255)  ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACgg                                                                          <  1:743381/80‑1 (MQ=255)  ccGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACgg                                                                          <  1:743380/80‑1 (MQ=255)   cGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGAc                                                                        <  2:743382/81‑1 (MQ=255)    gTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGAc                                                                        >  2:743433/1‑80 (MQ=255)    gTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGAc                                                                        >  1:743446/1‑80 (MQ=255)    gTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGAc                                                                        >  1:743421/1‑80 (MQ=255)     ttCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACg                                                                       >  2:743456/1‑80 (MQ=255)        aTACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAAt                                                                   >  1:743383/1‑81 (MQ=255)           cTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAAttt                                                                 <  2:743384/80‑1 (MQ=255)           cTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAAttt                                                                 <  2:743385/80‑1 (MQ=255)            tGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAc                                                               >  2:743432/1‑81 (MQ=255)            tGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAc                                                               >  1:743429/1‑81 (MQ=255)             gagaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAcc                                                              >  2:743457/1‑81 (MQ=255)              agaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAccc                                                             <  1:743388/81‑1 (MQ=255)              agaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAcc                                                              <  1:743386/80‑1 (MQ=255)              agaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAc                                                               <  1:743387/79‑1 (MQ=255)               gaTCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTAccc                                                             >  2:743460/1‑80 (MQ=255)                     gCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCa                                                           <  2:743389/76‑1 (MQ=255)                     gCGTGCTATAGGCCAGTACACAGACGATCCCAAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCgag                                                      <  2:743390/81‑1 (MQ=255)                      cGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCgaga                                                     <  1:743391/81‑1 (MQ=255)                       gTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGagag                                                    >  1:743438/1‑81 (MQ=255)                         gCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAgcg                                                  >  1:743452/1‑81 (MQ=255)                          cTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAgcgc                                                 <  1:743393/81‑1 (MQ=255)                          cTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAgcg                                                  <  1:743392/80‑1 (MQ=255)                              aGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTc                                             >  1:743442/1‑81 (MQ=255)                               ggCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTc                                             <  2:743394/80‑1 (MQ=255)                                 ccAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGAt                                          <  2:743395/81‑1 (MQ=255)                                 ccAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGAt                                          <  1:743396/81‑1 (MQ=255)                                  cAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATc                                         <  2:743397/81‑1 (MQ=255)                                      acacAGACGATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGc                                      >  2:743440/1‑80 (MQ=255)                                              gATCCCGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAAccc                             <  2:743398/81‑1 (MQ=255)                                                  ccGAATGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATg                         >  2:743447/1‑81 (MQ=255)                                                  ccGAATGGCAGTGCGCCAAACAGGACACACGGACGAAATTTAACAAAGCGAGAGCGCGTTCGATCGGCAAAAAAAACCAt                          <  2:743399/80‑1 (MQ=255)                                                     aaTGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGc                        >  2:743454/1‑79 (MQ=255)                                                      aTGGAAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAg                     <  1:743400/81‑1 (MQ=255)                                                       tGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAgg                    <  1:743402/81‑1 (MQ=255)                                                       tGGCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAgg                    <  1:743401/81‑1 (MQ=255)                                                         gCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGAt                  <  2:743403/81‑1 (MQ=255)                                                         gCAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGAt                  >  1:743450/1‑81 (MQ=255)                                                          cAGTGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATc                 <  2:743404/81‑1 (MQ=255)                                                             tGCGCCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCgg               >  2:743463/1‑80 (MQ=255)                                                              gcgcCAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGa              >  2:743464/1‑80 (MQ=255)                                                                  cAAACAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCg         >  1:743448/1‑81 (MQ=255)                                                                   aaaCAGTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCg         >  1:743462/1‑80 (MQ=255)                                                                     aCAGTACACACGGACGAAATTTACCCCAGCGGGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCAt      <  2:743405/81‑1 (MQ=255)                                                                        gTACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCa   <  1:743406/81‑1 (MQ=255)                                                                         tACACACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAg  <  2:743407/81‑1 (MQ=255)                                                                            |                                                                              GCCGTTCATACTGAGATCTGGCGTGCTATAGGCCAGTACACAGACGATCCCGAATGGCAGTGCGCCAAACAGTACCCACGGACGAAATTTACCCCAGCGAGAGCGCGTTCGATCGGCCAACAACCCCATGCAAGGATCGGAAATCGCATCCAG  >  NC_000913/3835609‑3835761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑