Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,843,177	G→A	H205Y (CAT→TAT)	adeQ ←	adenine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,843,177	0	G	A	100.0%	92.3 / NA	26	H205Y (CAT→TAT)	adeQ	adenine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/11);  total (15/11)

GACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACTTAAATCG  >  NC_000913/3843100‑3843253                                                                              |                                                                             gACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATaaaa                                                                           <  1:745077/81‑1 (MQ=255)    gccgcTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAAt                                                                         >  1:745112/1‑80 (MQ=255)      cgcTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGAc                                                                      <  2:745079/81‑1 (MQ=255)      cgcTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGAc                                                                      <  2:745078/81‑1 (MQ=255)       gcTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACg                                                                     <  1:745080/81‑1 (MQ=255)          aaaaTGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGtga                                                                  >  1:745108/1‑81 (MQ=255)               gAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGac                                                              >  1:745101/1‑80 (MQ=255)                        cGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTta                                                    <  2:745081/81‑1 (MQ=255)                            aaaTAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTataat                                                <  1:745082/81‑1 (MQ=255)                            aaaTAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTataa                                                 >  2:745111/1‑80 (MQ=255)                                 atCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATaaaa                                            >  1:745114/1‑80 (MQ=255)                                 atCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATaaaa                                            >  1:745107/1‑80 (MQ=255)                                 atCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATaaaa                                            >  1:745105/1‑80 (MQ=255)                                      caGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCt                                      <  1:745083/81‑1 (MQ=255)                                          aaGACGTCACCACAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTaaaa                                  <  1:745084/81‑1 (MQ=255)                                                 caccacAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTaa                           >  1:745117/1‑81 (MQ=255)                                                  accacAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTaac                          <  2:745085/81‑1 (MQ=255)                                                  accacAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTaa                           >  1:745113/1‑80 (MQ=255)                                                  accacAATAGAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTaa                           >  1:745120/1‑80 (MQ=255)                                                           gAAACCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCg                 >  1:745115/1‑81 (MQ=255)                                                             aaCCAGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTg               <  1:745086/81‑1 (MQ=255)                                                                 aGCACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGaa           >  2:745121/1‑81 (MQ=255)                                                                   cACCGCGGCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACt         <  1:745087/81‑1 (MQ=255)                                                                    aCCGCGGCATAAAAATGACGTGATGACAACACGGTTATATTAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACtt        <  2:869681/81‑1 (MQ=255)                                                                         ggCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACTTAAATc   >  2:745122/1‑81 (MQ=255)                                                                         ggCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACTTAAATc   >  2:745124/1‑81 (MQ=255)                                                                          gCATAAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACTTAAATCg  >  2:745119/1‑81 (MQ=255)                                                                              |                                                                             GACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTATAATAAAAAACCCTAAAATACCTAACAACACGCCGTGAGAACTTAAATCG  >  NC_000913/3843100‑3843253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑