Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,100	T→C	E562G (GAA→GGA)	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,100	0	T	C	100.0%	169.6 / NA	47	E562G (GAA→GGA)	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

GGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACAT  >  NC_000913/3851024‑3851175                                                                             |                                                                            ggTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATcccc                                                                          <  2:747265/80‑1 (MQ=255)    cATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCccac                                                                      >  2:747341/1‑81 (MQ=255)     aTTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCccacc                                                                     <  1:747266/81‑1 (MQ=255)      ttACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCccacca                                                                    >  2:747313/1‑81 (MQ=255)        aCGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCAt                                                                   <  2:747267/80‑1 (MQ=255)         cGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCAttt                                                                 <  2:747268/81‑1 (MQ=255)          gCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCAttt                                                                 >  1:747325/1‑80 (MQ=255)          gCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTc                                                                <  1:747269/81‑1 (MQ=255)          gCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTc                                                                <  2:747270/81‑1 (MQ=255)           cccGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCAttt                                                                 >  2:747323/1‑79 (MQ=255)           cccGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCa                                                               <  2:747271/81‑1 (MQ=255)                 tGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTc                                                         <  1:747272/81‑1 (MQ=255)                   gTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTcgc                                                       >  2:747321/1‑81 (MQ=255)                      gCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTcgccg                                                     <  1:747273/80‑1 (MQ=255)                       cGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTcgccgc                                                    <  1:747274/80‑1 (MQ=255)                        gAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCAc                                                  <  2:747275/81‑1 (MQ=255)                         aaCGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCAcc                                                 <  1:747276/81‑1 (MQ=255)                         aaCGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCAcc                                                 >  2:747333/1‑81 (MQ=255)                          aCGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCt                                                >  2:747315/1‑81 (MQ=255)                           cGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTg                                               <  2:747277/81‑1 (MQ=255)                            ggTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTgg                                              <  1:747278/81‑1 (MQ=255)                            ggTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTgg                                              >  2:747330/1‑81 (MQ=255)                             gTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTggc                                             <  1:747279/81‑1 (MQ=255)                                aGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTggcggc                                          <  2:747280/81‑1 (MQ=255)                                  cTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCAc                                        >  1:747329/1‑81 (MQ=255)                                  cTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCAc                                        <  1:747281/81‑1 (MQ=255)                                   tCCAGAATTACGTTTTCATGAGTTCTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATtcgcctcacctcgcctcacc                                       >  2:747334/1‑81 (MQ=255)                                       gAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCg                                   >  2:747343/1‑81 (MQ=255)                                          ttACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGt                                <  2:747282/81‑1 (MQ=255)                                           tACGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGt                                >  1:747332/1‑80 (MQ=255)                                            aCGTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTa                               >  2:747353/1‑80 (MQ=255)                                              gTTGTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAAc                            >  2:747352/1‑81 (MQ=255)                                                 gTCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACttt                         >  2:747324/1‑81 (MQ=255)                                                  tCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACtttt                        <  2:747283/81‑1 (MQ=255)                                                  tCATGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACttt                         <  2:747284/80‑1 (MQ=255)                                                    aTGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTttc                      <  2:747285/81‑1 (MQ=255)                                                    aTGAGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTttc                      >  1:747336/1‑81 (MQ=255)                                                       aGTTGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTttctt                    <  1:747286/80‑1 (MQ=255)                                                         ttGTGTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCgg                 >  1:747326/1‑81 (MQ=255)                                                          tgtgTTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCgg                 >  1:747335/1‑80 (MQ=255)                                                           gtgtTTTGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGc                <  2:747287/80‑1 (MQ=255)                                                                ttGCATGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCaa           <  1:747288/80‑1 (MQ=255)                                                                    aTGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATgcg       >  2:747331/1‑80 (MQ=255)                                                                     tGGCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGca     >  1:747348/1‑81 (MQ=255)                                                                      ggCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGca     >  1:747342/1‑80 (MQ=255)                                                                       gCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGcaca   <  2:747290/81‑1 (MQ=255)                                                                       gCTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGcac    <  2:747289/80‑1 (MQ=255)                                                                        cTTATCCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACAt  >  2:747350/1‑81 (MQ=255)                                                                             |                                                                            GGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACCATCGGGTAAACTTTTTCTTCGGCATCAATGCGCACAT  >  NC_000913/3851024‑3851175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑