Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,855,366	A→T	S82R (AGT→AGA)	yidF ←	uncharacterized protein YidF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,855,366	0	A	T	100.0%	120.4 / NA	34	S82R (AGT→AGA)	yidF	uncharacterized protein YidF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/21);  total (13/21)

GCAGTCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGACTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGCGGC  >  NC_000913/3855288‑3855443                                                                               |                                                                              gCAGTCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCt                                                                             <  1:748409/81‑1 (MQ=255)   aGTCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGa                                                                           >  2:748471/1‑81 (MQ=255)    gTCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGat                                                                          <  1:748410/81‑1 (MQ=255)     tCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGata                                                                         <  2:748411/81‑1 (MQ=255)       acaGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATc                                                                       >  2:748474/1‑81 (MQ=255)          gcgcGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGc                                                                    <  1:748412/81‑1 (MQ=255)          gcgcGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGc                                                                    <  1:748413/81‑1 (MQ=255)            gcgGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGccc                                                                  <  2:748414/81‑1 (MQ=255)              ggTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGc                                                                <  2:748415/81‑1 (MQ=255)                   cGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCa                                                           <  1:748417/81‑1 (MQ=255)                   cGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCa                                                           <  2:748416/81‑1 (MQ=255)                      gTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAaca                                                        >  2:748466/1‑81 (MQ=255)                      gTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAaca                                                        <  2:748418/81‑1 (MQ=255)                         ttCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACt                                                      <  1:748419/80‑1 (MQ=255)                          tCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTc                                                     <  1:748421/80‑1 (MQ=255)                          tCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTc                                                     <  1:748420/80‑1 (MQ=255)                          tCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTc                                                     >  1:748473/1‑80 (MQ=255)                           cGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCg                                                    <  2:748422/80‑1 (MQ=255)                                  acacCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGc                                            <  2:748423/81‑1 (MQ=255)                                   cacCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCa                                           <  1:748424/81‑1 (MQ=255)                                     ccATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAAt                                         <  1:748425/81‑1 (MQ=255)                                              tAAGGTGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAAttt                                >  1:748462/1‑81 (MQ=255)                                                   tGGCGTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTcccc                            >  1:748475/1‑80 (MQ=255)                                                       gTCAAAAAGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAg                       >  2:748468/1‑81 (MQ=255)                                                          aaaaaGCTCAATCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGctc                    >  2:748479/1‑81 (MQ=255)                                                                   aaTCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTc             <  2:748427/79‑1 (MQ=255)                                                                   aaTCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCa            <  2:748426/80‑1 (MQ=255)                                                                   aaTCGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCAc           >  1:748464/1‑81 (MQ=255)                                                                      cGAGATACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCg         >  2:748488/1‑80 (MQ=255)                                                                       gagaTACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGaa       <  2:748428/81‑1 (MQ=255)                                                                         gaTACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGc     >  2:748470/1‑81 (MQ=255)                                                                          aTACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGCg    >  2:748469/1‑81 (MQ=255)                                                                           tACGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGCgg   <  1:748429/81‑1 (MQ=255)                                                                            aCGTCTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGCGGc  >  2:748485/1‑81 (MQ=255)                                                                               |                                                                              GCAGTCACAGCGCGGTTGCGGGTTTTCGCTGCCACACCATTTTTGTAAGGTGGCGTCAAAAAGCTCAATCGAGATACGACTGATATCTGCCCGCACCCAACACTCGAACAGGGCAATTAAAAATTTCCCCCACAGCTCTGCTGTCACCGAAGCGGC  >  NC_000913/3855288‑3855443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑