Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	394,441	C→T	intergenic (+23/‑65)	yaiT → / → iprA	putative autotransporter YaiT/putative DNA‑binding transcriptional regulator IprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	394,441	0	C	T	100.0%	42.6 / NA	13	intergenic (+23/‑65)	yaiT/iprA	putative autotransporter YaiT/putative DNA‑binding transcriptional regulator IprA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

GTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTT  >  NC_000913/394371‑394510                                                                       |                                                                      gTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTa                                                             <  1:87128/81‑1 (MQ=255)     aTCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCt                                                         <  1:87129/81‑1 (MQ=255)              ggTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGc                                                >  1:87157/1‑81 (MQ=255)                          tGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGAtt                                    <  1:87130/81‑1 (MQ=255)                          tGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGAtt                                    >  1:87160/1‑81 (MQ=255)                              tgtTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGAc                                <  2:87131/81‑1 (MQ=255)                                      ataCCTGGTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTa                        >  1:87159/1‑81 (MQ=255)                                            ggTAATATTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCa                   >  1:87153/1‑80 (MQ=255)                                                atatTCTTCACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGa              <  2:87132/81‑1 (MQ=255)                                                   ttcttcACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGAcgc           <  1:87133/81‑1 (MQ=255)                                                   ttcttcACTCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGAcgc           <  1:87134/81‑1 (MQ=255)                                                           tCCGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGt   >  2:87164/1‑81 (MQ=255)                                                             cGAAGAAATATTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGtt  <  2:87135/80‑1 (MQ=255)                                                                       |                                                                      GTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTT  >  NC_000913/394371‑394510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑